A transcriptomic analysis of Chrysanthemum nankingense provides insights into the basis of low temperature tolerance
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  • 作者：Liping Ren (2) (3)
    Jing Sun (2)
    Sumei Chen (2)
    Jiaojiao Gao (2)
    Bin Dong (2)
    Yanan Liu (2)
    Xiaolong Xia (2)
    Yinjie Wang (2)
    Yuan Liao (2)
    Nianjun Teng (2)
    Weimin Fang (2)
    Zhiyong Guan (2)
    Fadi Chen (2) (3)
    Jiafu Jiang (2)

    2. College of Horticulture     ; Nanjing Agricultural University ; Nanjing ; 210095 ; China
    3. Jiangsu Province Engineering Lab for Modern Facility Agriculture Technology & Equipment     ; No. 1 Weigang ; Nanjing ; 210095 ; Jiangsu Province ; China
  • 关键词：Transcriptome ; RNA Sequencing ; Low temperature tolerance ; Ornamental plant
  • 刊名：BMC Genomics
  • 出版年：2014
  • 出版时间：December 2014
  • 年：2014
  • 卷：15
  • 期：1
  • 全文大小：931 KB
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  • 刊物主题：Life Sciences, general; Microarrays; Proteomics; Animal Genetics and Genomics; Microbial Genetics and Genomics; Plant Genetics & Genomics;
  • 出版者：BioMed Central
  • ISSN：1471-2164
文摘
Background A major constraint affecting the quality and productivity of chrysanthemum is the unusual period of low temperature occurring during early spring, late autumn, and winter. Yet, there has been no systematic investigation on the genes underlying the response to low temperature in chrysanthemum. Herein, we used RNA-Seq platform to characterize the transcriptomic response to low temperature by comparing different transcriptome of Chrysanthemum nankingense plants and subjecting them to a period of sub-zero temperature, with or without a prior low temperature acclimation. Results Six separate RNA-Seq libraries were generated from the RNA samples of leaves and stems from six different temperature treatments, including one cold acclimation (CA), two freezing treatments without prior CA, two freezing treatments with prior CA and the control. At least seven million clean reads were obtained from each library. Over 77% of the reads could be mapped to sets of C. nankingense unigenes established previously. The differentially transcribed genes (DTGs) were identified as low temperature sensing and signalling genes, transcription factors, functional proteins associated with the abiotic response, and low temperature-responsive genes involved in post-transcriptional regulation. The differential transcription of 15 DTGs was validated using quantitative RT-PCR. Conclusions The large number of DTGs identified in this study, confirmed the complexity of the regulatory machinery involved in the processes of low temperature acclimation and low temperature/freezing tolerance.

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