Comparative analysis of gene expression profiles of OPN signalling pathway in four kinds of liver diseases
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- 作者：GAIPING WANG ; SHASHA CHEN ; CONGCONG ZHAO ; XIAOFANG LI ; WEIMING ZHAO…
- 关键词：osteopontin signalling pathway ; nonalcoholic fatty liver disease ; liver cirrhosis ; hepatic cancer ; acute hepatic failure.
- 刊名：Journal of Genetics
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：95
- 期：3
- 页码：741-750
- 全文大小：2,345 KB
- 刊物主题：Life Sciences, general; Microbial Genetics and Genomics; Plant Genetics & Genomics; Animal Genetics and Genomics; Evolutionary Biology;
- 出版者：Springer India
- ISSN：0973-7731
- 卷排序：95
文摘
To explore the relevance of OPN signalling pathway to the occurrence and development of nonalcoholic fatty liver disease (NAFLD), liver cirrhosis (LC), hepatic cancer (HC) and acute hepatic failure (AHF) at transcriptional level, Rat Genome 230 2.0 Array was used to detect expression profiles of OPN signalling pathway-related genes in four kinds of liver diseases. The results showed that 23, 33, 59 and 74 genes were significantly changed in the above four kinds of liver diseases, respectively. H-clustering analysis showed that the expression profiles of OPN signalling-related genes were notably different in four kinds of liver diseases. Subsequently, a total of above-mentioned 147 genes were categorized into four clusters by k-means according to the similarity of gene expression, and expression analysis systematic explorer (EASE) functional enrichment analysis revealed that OPN signalling pathway-related genes were involved in cell adhesion and migration, cell proliferation, apoptosis, stress and inflammatory reaction, etc. Finally, ingenuity pathway analysis (IPA) software was used to predict the functions of OPN signalling-related genes, and the results indicated that the activities of ROS production, cell adhesion and migration, cell proliferation were remarkably increased, while that of apoptosis, stress and inflammatory reaction were reduced in four kinds of liver diseases. In summary, the above physiological activities changed more obviously in LC, HC and AHF than in NAFLD.