Isolation, characterization, and expression analysis of CmMLO2 in muskmelon

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• 作者：Hong Cheng (1)
  Weiping Kong (1)
  Dong Hou (1)
  Junfeng Lv (2)
  Xinglin Tao (1)
  
• 关键词：Cloning ; Expression ; Melon ; MLO
• 刊名：Molecular Biology Reports
• 出版年：2013
• 出版时间：March 2013
• 年：2013
• 卷：40
• 期：3
• 页码：2609-2615
• 全文大小：480KB
• 参考文献：1. Panstruga R (2005) Serpentine plant MLO proteins as entry portals for powdery mildew fungi. Biochem Soc Trans 33:389-92 CrossRef
  2. Freialdenhoven A, Peterha¨nsel C, Kurth J, Kreuzaler F, Schulze-Lefert P (1996) Identification of genes required for the function of non-race-specific mlo resistance to powdery mildew in barley. Plant Cell 8:5-4
  3. Piffanelli P, Zhou F, Casais C, Orme J, Jarosch B, Schaffrath U, Collins N, Panstruga R, Schulze-Lefert P (2002) The Barley MLO modulator of defense and cell death is responsive to biotic and abiotic stress Stimuli. Plant Physiol 129:1076-085 CrossRef
  4. J?rgensen JH (1992) Discovery, characterization and exploitation of Mlo powdery mildew resistance in barley. Euphytica 63:141-52 CrossRef
  5. Büschges R et al (1997) The barley Mlo gene: a novel control element of plant pathogen resistance. Cell 88:695-05 CrossRef
  6. Kim Min C, Panstruga Ralph, Elliott Candace (2002) Calmodulin interacts with MLO protein to regulate defence against mikfre in barley. Nature 416:447-50 CrossRef
  7. Panstruga R, Schulze-Lefert P (2003) Corruption of host seven-transmembrane proteins by pathogenic microbes: a common theme in animals and plants? Microbes Infect 5:429-37 CrossRef
  8. Liu Q, Zhu H (2008) Molecular evolution of the MLO gene family in / Oryza / sativa and their functional divergence. Gene 409:1-0 CrossRef
  9. Devoto A, Hartmann HA, Piffanelli P, Elliott C, Simmons C, Taramino G, Goh CS, Cohen FE, Emerson BC, Schulze-Lefert P, Panstruga R (2003) Molecular phylogeny and evolution of the plant-specific seven-transmembrane MLO family. J Mol Evol 56:77-8 CrossRef
  10. Devoto A et al (1999) Topology, subcellular localization, and sequence diversity of the Mlo family in plants. J Biol Chem 274:34993-5004 CrossRef
  11. Hong C, Qiwei H, Yumeng H, Lixia H, Junfen L (2009) Molecular cloning, characterization and expression analysis of / CmAPX. Mol Biol Rep 36(6):1531-537 CrossRef
  12. Hong C, Weiping K, Dongshun L, Yongquan S, Qiwei H (2012) Molecular cloning and expression analysis of CmMlo1 in melon. Mol Biol Rep 39(2):1903-907 CrossRef
  13. Sun W, Cao Z, Li Y, Zhao Y, Zhang H (2007) A simple and effective method for protein subcellular localization using / Agrobacterium -mediated transformation of onion epidermal cells. Biologia 62:529-32 CrossRef
  14. Consonni C, Humphry ME, Hartmann HA, Livaja M, Durner J, Westphal L, Vogel J, Lipka V, Kemmerling B, Schulze-Lefert P, Somerville SC, Panstruga R (2006) Conserved requirement for a plant host cell protein in powdery mildew pathogenesis. Nat Genet 38:716-20 CrossRef
  15. Kim M, Panstruga R, Elliott C, Müller J, Devoto A, Yoon H, Park H, Cho M, Schulze-Lefert P (2002) Calmodulin interacts with MLO protein to regulate defence against mildew in barley. Nat a–z Index 416:447-51 CrossRef
  16. Chen Z, Hartmann HA, Wu MJ, Friedman EJ, Chen JG, Pulley M, Schulze-Lefert P, Panstruga R, Jones AM (2006) Expression analysis of the AtMLO gene family encoding plant-specific seven-transmembrane domain proteins. Plant Mol Biol 60:583-97 CrossRef
  
• 作者单位：Hong Cheng (1)
  Weiping Kong (1)
  Dong Hou (1)
  Junfeng Lv (2)
  Xinglin Tao (1)
  
  1. Institute of Vegetable, Gansu Academy of Agricultural Science, Lanzhou, 730070, China
  2. College of Veterinary Medicine, Gansu Agricultural Universities, Lanzhou, 730070, China
  
• ISSN：1573-4978

文摘

The full-length cDNA sequence of the MLO gene was cloned via SMART-RACE-PCR from muskmelon (Cucumis melo L.), and was designated as CmMLO2 (GenBank Accession No. FJ713542). The gene is 1,710?bp long and encodes a 570-amino acid peptide with a seven-transmembrane domain topology, and is a typical transmembrane protein. Localization analysis in onion epidermal cells showed that CmMLO2-GFP is localized in the plasma membrane. The expression of CmMLO2 gene was analyzed in melon leaf infected with powdery mildew using a quantitative RT-PCR and it was found that CmMLO2 was mainly expressed in melon leaves in a no-tissue-specific pattern. Moreover, CmMLO2 may play a crucial role in the pathogenesis of powdery mildew.