GmPRP2 promoter drives root-preferential expression in transgenic Arabidopsis and soybean hairy roots
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- 作者：Li Chen ; Bingjun Jiang ; Cunxiang Wu ; Shi Sun ; Wensheng Hou ; Tianfu Han
- 关键词：Soybean ; PRP2 ; Promoter ; Root ; preferential ; Arabidopsis thaliana ; Deletion ; GUS ; Hairy root
- 刊名：BMC Plant Biology
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：14
- 期：1
- 全文大小：3,478 KB
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- 出版者：BioMed Central
- ISSN：1471-2229
文摘
Background Promoters play important roles in gene expression and function. There are three basic types of promoters: constitutive, specific, and inducible. Constitutive promoters are widely used in genetic engineering, but these promoters have limitations. Inducible promoters are activated by specific inducers. Tissue-specific promoters are a type of specific promoters that drive gene expression in specific tissues or organs. Here, we cloned and characterized the GmPRP2 promoter from soybean. The expression pattern indicated that this promoter is root-preferential in transgenic Arabidopsis and the hairy roots of soybean. It can be used to improve the root resistance or tolerance to pathogens, pests, malnutrition and other abiotic stresses which cause extensive annual losses in soybean production. Results The GmPRP2 promoter (GmPRP2p-1062) was isolated from soybean cv. Williams 82. Sequence analysis revealed that this promoter contains many cis-acting elements, including root-specific motifs. The GmPRP2p-1062 and its 5-deletion fragments were fused with the GUS reporter gene and introduced into Arabidopsis and the hairy roots of soybean to further determine promoter activity. Histochemical analysis in transgenic Arabidopsis showed that GUS activity was mainly detected in roots and hypocotyls in all deletion fragments except GmPRP2p-471 (a 5-deletion fragment of GmPRP2p-1062 with 471?bp length). GUS activity was higher in transgenic Arabidopsis and hairy roots with GmPRP2p-1062 and GmPRP2p-852 (a 5-deletion fragment of GmPRP2p-1062 with 852?bp length) constructs than the other two constructs. GUS activity was enhanced by NaCl, PEG, IAA and JM treatments and decreased by SA, ABA and GA treatments in transgenic Arabidopsis. Conclusions GmPRP2p-1062 is a root-preferential promoter, and its core fragment for root-preferential expression might lie between ?69 and +1. GmPRP2p-852 may be useful in the genetic engineering of novel soybean cultivars in the future.