Overexpression of a SNARE protein AtBS14b alters BR response in Arabidopsis

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• 作者：Zhong Xin Zhu (1)
  Hong Bo Ye (3)
  Yuan Hu Xuan (2)
  Da Nian Yao (1)
  
  1. Agricultural College ; Anhui Agricultural University ; Changjiangxi Road 130 ; Hefei ; Anhui ; 230036 ; China
  3. Department of Biology ; Stanford University ; N. Service Road ; Stanford ; CA ; 94305-5020 ; USA
  2. College of Pharmaceutical Sciences ; Wenzhou Medical University ; Xueyuanxi Road 82 ; Wenzhou ; Zhejiang ; 325035 ; China
  
• 关键词：SNARE ; Trafficking ; BR ; MSBP1 ; Arabidopsis
• 刊名：Botanical Studies
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：55
• 期：1
• 全文大小：3,423 KB
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• 刊物主题：Plant Sciences;
• 出版者：Springer Berlin Heidelberg
• ISSN：1999-3110

文摘

Background N-ethyl-maleimide sensitive factor adaptor protein receptor (SNAREs) domain-containing proteins were known as key players in vesicle-associated membrane fusion. Genetic screening has revealed the function of SNAREs in different aspects of plant biology, but the role of many SNAREs are still unknown. In this study, we have characterized the role of Arabidopsis Qc-SNARE protein AtBS14b in brassinosteroids (BRs) signaling pathway. Results AtBS14b overexpression (AtBS14b ox) plants exhibited short hypocotyl and petioles lengths as well as insensitivity to exogenously supplied BR, while AtBS14b mutants did not show any visible BR-dependent morphological differences. BR biosynthesis enzyme BR6OX2 expression was slightly lower in AtBS14b ox than in wild type plants. Further BR-mediated repression of BR6OX2, CPD and DWF4 was inhibited in AtBS14b ox plants. AtBS14b-mCherry fusion protein localized in vesicular compartments surrounding plasma membrane in N. benthamiana leaves. In addition, isolation of AtBS14b-interacting BR signaling protein, which localized in plasma membrane, showed that AtBS14b directly interacted with membrane steroid binding protein 1 (MSBP1), but did not interact with BAK1 or BRI1. Conclusion These data suggested that Qc-SNARE protein AtBS14b is the first SNARE protein identified that interacts with MSBP1, and the overexpression of AtBS14b modulates BR response in Arabidopsis.