Molecular characteristics of three thymosin-repeat proteins from Marsupenaeus japonicus and their responses to WSSV infection

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• 作者：Jinyou Ma ; Lingwei Ruan ; Xun Xu ; Zhaoming Gao
• 关键词：β ; thymosin ; thymosin ; repeat proteins ; white spot syndrome virus (WSSV) ; Marsupenaeus japonicus
• 刊名：Acta Oceanologica Sinica
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：35
• 期：4
• 页码：44-50
• 全文大小：450 KB
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• 作者单位：Jinyou Ma (1) (2) (3)
  Lingwei Ruan (2) (3)
  Xun Xu (2) (3)
  Zhaoming Gao (2) (3)
  
  1. College of Animal Sciences, Henan Institute of Science and Technology, Xinxiang, 453003, China
  2. State Key Laboratory Breeding Base of Marine Genetic Resources, Xiamen, 361005, China
  3. Key Laboratory of Marine Genetic Resources of State Oceanic Administration, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, China
  
• 刊物主题：Oceanography; Climatology; Ecology; Engineering Fluid Dynamics; Marine & Freshwater Sciences; Environmental Chemistry;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-1099

文摘

β-thymosins, a family of highly conserved peptides, play a vital role in wound-healing, angiogenesis, antimicrobial process and antiviral immunity. Three novel β-thymosin-repeat proteins, named mjthm4, mjthm3 and mjthm2, were cloned from Marsupenaeus japonicus using expressed sequence tags (EST) from suppression subtractive hybridization. The mjthm4, mjthm3 and mjthm2 cDNAs possessed open reading frames that encoded 166, 128 and 90 amino acid residue polypeptides and contained four, three and two β-thymosin actin binding modules, respectively. Blast analysis demonstrated that mjthm4, mjthm3 and mjthm2 shared high homology with known invertebrate multi-repeat β-thymosins. These proteins are ubiquitously expressed in all of the examined tissues, and the transcriptional levels were highest in the intestine. Further investigation revealed that mjthm4, mjthm3 and mjthm2 were remarkably up-regulated 6 h after WSSV infection. Moreover, while mjthm4 transcriptional levels displayed no changes, mjthm3 and mjthm2 levels decreased in the virus-resistant shrimps. The results indicate that mjthm4, mjthm3 and mjthm2 are novel multi-repeat β-thymosin homologues, have a close relationship with WSSV infection, and might contribute to a better understanding of host defense and/or virus invasion interactions in shrimps.