Phage display for identifying peptides that bind the spike protein of transmissible gastroenteritis virus and possess diagnostic potential

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• 作者：Siqingaowa Suo ; Xue Wang ; Dante Zarlenga ; Ri-e Bu ; Yudong Ren ; Xiaofeng Ren
• 关键词：TGEV ; rS ; AD protein ; Peptide ; Diagnosis ; Spike protein
• 刊名：Virus Genes
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：51
• 期：1
• 页码：51-56
• 全文大小：766 KB
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• 作者单位：Siqingaowa Suo (1) (2)
  Xue Wang (1)
  Dante Zarlenga (3)
  Ri-e Bu (2)
  Yudong Ren (1)
  Xiaofeng Ren (1) (2)
  
  1. Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin, 150030, China
  2. College of Life Science, Inner Mongolia University for Nationalities, No. 866, Xilamulun Street, Tong Liao, 028043, Inner Mongolia, China
  3. Animal Parasitic Diseases Laboratory, Agricultural Research Service, Beltsville, MD, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Virology
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-994X

文摘

The spike (S) protein of porcine transmissible gastroenteritis virus (TGEV) is located within the viral envelope and is the only structural protein that possesses epitopes capable of inducing virus-neutralizing antibodies. Among the four N-terminal antigenic sites A, B, C, and D, site A and to a lesser extent site D (S-AD) induce key neutralizing antibodies. Recently, we expressed S-AD (rS-AD) in recombinant form. In the current study, we used the rS-AD as an immobilized target to identify peptides from a phage-display library with application for diagnosis. Among the 9 phages selected that specifically bound to rS-AD, the phage bearing the peptide TLNMHLFPFHTG bound with the highest affinity and was subsequently used to develop a phage-based ELISA for TGEV. When compared with conventional antibody-based ELISA, phage-mediated ELISA was more sensitive; however, it did not perform better than semi-quantitative RT-PCR, though phage-mediated ELISA was quicker and easier to set up.