Doxorubicin Induces Sarcoplasmic Reticulum Calcium Regulation Dysfunction via the Decrease of SERCA2 and Phospholamban Expressions in Rats
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- 作者：Yachen Zhang (1)
Yu Chen (1)
Min Zhang (1)
Yong Tang (1)
Yuquan Xie (1)
Xiaohong Huang (1)
Yigang Li (1) - 关键词：Doxorubicin ; Sarcoplasmic reticulum calcium regulation dysfunction ; SERCA2 ; Phospholamban
- 刊名：Cell Biochemistry and Biophysics
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：70
- 期：3
- 页码：1791-1798
- 全文大小：708 KB
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Yu Chen (1)
Min Zhang (1)
Yong Tang (1)
Yuquan Xie (1)
Xiaohong Huang (1)
Yigang Li (1)
1. Division of Cardiology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, 1665 Kong Jiang Road, Shanghai, 200092, China - ISSN：1559-0283
文摘
This study aims to explore the changes in calcium regulation in the sarcoplasmic reticulum (SR) during doxorubicin (DOX)?treatment. Sprague–Dawley rats were treated with intravenous DOX (1.5?mg/kg) twice weekly for 12 treatments. The hemodynamic changes, myocardial oxidative stress, levels of cardiac toxicity markers, and calcium handling of the myocardial SR were observed. When the accumulation of DOX reached 12?mg/kg, (1) heart weight, left ventricular mass, and lung congestion increased significantly, and ascites appeared; (2) SBP, DBP, MAP, +dP/dt, ?dP/dt, and LVSP decreased significantly, and LVEDP increased (p?iNOS activity and MDA and NO concentrations significantly increased, while the SOD decreased (p?p?2+ absorption function and Ca2+-stimulated ATPase activity declined dramatically, as did the SERCA2 and phospholamban levels (p?p?