Bone marrow enriched graft, modified by self-assembly peptide, repairs critically-sized femur defects in goats
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- 作者：Zhiqiang Li (1) (2) (3) (4)
Tianyong Hou (1) (2) (3)
Fei Luo (1) (2) (3)
Zhengqi Chang (1) (2) (3)
Xuehui Wu (1) (2) (3)
Junchao Xing (1) (2)
Moyuan Deng (1) (2) (3)
Jianzhong Xu (1) (2) (3) - 关键词：Bone marrow ; Self ; assembly peptide ; Enriched graft ; Critically ; sized defect
- 刊名：International Orthopaedics
- 出版年：2014
- 出版时间：November 2014
- 年：2014
- 卷：38
- 期：11
- 页码：2391-2398
- 全文大小：1,592 KB
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17. Place ES, Evans ND, Stevens MM (2009) Complexity in biomaterials for tissue engineering. Nat Mater 8(6):457-70. doi:10.1038/nmat2441 Xuehui Wu (1) (2) (3)
Junchao Xing (1) (2)
Moyuan Deng (1) (2) (3)
Jianzhong Xu (1) (2) (3)
1. National & Regional United Engineering Lab of Tissue Engineering, Department of Orthopaedics, Southwest Hospital, The Third Military Medical University, Chongqing, China
2. Center of Regenetive and Reconstructive Engineering, Technology in Chongqing City, Chongqing, China
3. Tissue Engineering Laboratory of Chongqing City, Chongqing, China
4. Department of Orthopaedics, General Hospital of Chengdu Military Region, Chengdu, China - ISSN：1432-5195
文摘
Purpose This study focuses on nanoscale self–assembly peptides (SAP) modified demineralized bone matrix (DBM) which provided a more effective osteogenesis and regeneration for critically-sized femur defects in goats using the selective cell retention (SCR) strategy. Methods RADA16–I peptide was used to modify DBM and formed a composite scaffold (SAP/DBM). The morphological change and dynamic expression of osteogenic genes of mesenchymal stem cells (MSCs) derived from marrow in SAP/DBM was observed. The cells and factors in bone marrow were enriched into SAP/DBM by technology of selective cells retension (SCR). The construct was transplanted into 20-mm femur defects in goats and their osteogenesis was evaluated. Results The SAP/DBM scaffold formed a three-dimensional interweaving nanofiber in pores of DBM. MSCs exhibited better morphology in SAP/DBM than that in only DBM, and the levels of expression of ALP ,OCN and Runx2 gene in SAP/DBM samples was significantly higher than that of DBM at 14?days in vitro (P--.05). Compared with marrow-enriched DBM, the volume of newly formed bone from marrow-enriched SAP/DBM is higher in goats (P--.05). Conclusion Our study may not only have a significant impact on the construction method of tissue engineering but also provide a viable, simple and effective method for clinical bone construction.