Inhibition of autophagy induced by TSA sensitizes colon cancer cell to radiation

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• 作者：Gang He (1)
  Yan Wang (1)
  Xueli Pang (2)
  Bo Zhang (1)
  
• 关键词：TSA ; Colon cancer ; Radiosensitivity ; Autophagy ; Apoptosis
• 刊名：Tumor Biology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：35
• 期：2
• 页码：1003-1011
• 全文大小：473 KB
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• 作者单位：Gang He (1)
  Yan Wang (1)
  Xueli Pang (2)
  Bo Zhang (1)
  
  1. Department of Medical Genetics, Third Military Medical University, Chongqing, 400038, China
  2. Department of oncology, Southwest Hospital, Third Military Medical University, Chongqing, China
  
• ISSN：1423-0380

文摘

Radiotherapy is one of the main treatments for clinical cancer therapy. However, its application was limited due to lack of radiosensitivity in some cancers. Trichostatin A (TSA) is a classic histone deacetylases inhibitor (HDACi) that specifically inhibits the biochemical functions of HDAC and is demonstrated to be an active anticancer drug. However, whether it could sensitize colon cancer to radiation is not clear. Our results showed that TSA enhanced the radiosensitivity of colon cancer cells as determined by CCK-8 and clonogenic survival assay. Moreover, apoptotic cell death induced by radiation was enhanced by TSA treatment. Additionally, TSA also induced autophagic response in colon cancer cells, while autophagy inhibition led to cell apoptosis and enhanced the radiosensitivity of colon cancer cells. Our data suggested that inhibition of cytoprotective autophagy sensitizes cancer cell to radiation, which might be further investigated for clinical cancer radiotherapy.