Detection and quantification of airborne inoculum of Blumeria graminis f. sp. tritici using quantitative PCR

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• 作者：Xueren Cao ; Dongming Yao ; Yilin Zhou…
• 刊名：European Journal of Plant Pathology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：146
• 期：1
• 页码：225-229
• 全文大小：525 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Pathology
  Plant Sciences
  Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-8469
• 卷排序：146

文摘

In this study, a real-time PCR assay was established to quantify airborne inoculum of Blumeria graminis f. sp. tritici (Bgt), the causal agent of wheat powdery mildew. Burkard 7-day recording spore traps were used to collect Bgt spores in air in Langfang City, Hebei Province, China. A linear relationship between number of spores counted with a compound microscope and those determined with the real-time PCR assay was obtained in artificially inoculated spore trap tape sections. The dynamics of airborne conidia concentrations of Bgt were similar. There were significant correlations (P < 0.01) between spore concentrations by using a compound microscope and the real-time PCR assay in three growing seasons. The results demonstrated a potential method to quantitatively determine spore inoculum potential in wheat fields by using a real-time PCR assay.KeywordsBlumeria graminis f. sp. triticiWheat powdery mildewReal-time PCRAirborne conidiaSpore trap