Matrine inhibits proliferation and induces apoptosis of human colon cancer LoVo cells by inactivating Akt pathway
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- 作者：Shujun Zhang (1)
Binglin Cheng (2)
Hali Li (3)
Wei Xu (2)
Bo Zhai (3)
Shangha Pan (3)
Lei Wang (4)
Ming Liu (4)
Xueying Sun (3) - 关键词：Matrine ; LoVo cells ; Apoptosis ; Cell cycle ; Akt pathway
- 刊名：Molecular Biology Reports
- 出版年：2014
- 出版时间：April 2014
- 年：2014
- 卷：41
- 期：4
- 页码：2101-2108
- 全文大小：1,013 KB
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Binglin Cheng (2)
Hali Li (3)
Wei Xu (2)
Bo Zhai (3)
Shangha Pan (3)
Lei Wang (4)
Ming Liu (4)
Xueying Sun (3)
1. Department of Pathology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150001, China
2. Department of Integrated Medicine, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China
3. The Hepatosplenic Surgery Center, Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, 150001, China
4. Department of Oncology, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, 150001, China - ISSN：1573-4978
文摘
The present study has investigated the anti-tumor activity and the underlying mechanisms of matrine on human colon cancer LoVo cells. Matrine inhibited the proliferation of the cells in dose- and time-dependent manners. The concentration required for 50?% inhibition (IC50) was 1.15, 0.738, and 0.414?mg/ml, when cell were incubated with matrine for 24, 48, and 72?h, respectively. Matrine induced cell cycle arrest at G1 phase by downregulating cyclin D1 and upregulating p27 and p21. Matrine induced cell apoptosis by reducing the ratio of Bcl-2/Bax and increasing the activation of caspase-9 in a dose-dependent manner. Matrine displayed its anti-tumor activity by inactivating Akt, the upstream factor of the above proteins. Matrine significantly reduced the protein levels of pAkt, and increased the protein levels of other downstream factors, pBad and pGSK-3β. Specific inhibition of pAkt induced cell apoptosis, and synergized with matrine to inhibit the proliferation of LoVo cells; whereas activation of Akt neutralized the inhibitory effect of matrine on cell proliferation. The present study has demonstrated that matrine inhibits proliferation and induces apoptosis of human colon cancer LoVo cells by inactivating Akt pathway, indicating matrine may be a potential anti-cancer agent for colon cancer.