Lesional infiltration of mast cells, Langerhans cells, T cells and local cytokine profiles in alopecia areata

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• 作者：Xiaoting Zhang ; Ying Zhao ; Yanting Ye ; Shuifeng Li…
• 关键词：Alopecia areata ; Histopathology ; Mast cell ; Langerhans cell ; T lymphocyte ; Cytokines ; Immunohistochemistry ; Semi ; quantitative real time PCR
• 刊名：Archives of Dermatological Research
• 出版年：2015
• 出版时间：May 2015
• 年：2015
• 卷：307
• 期：4
• 页码：319-331
• 全文大小：1,806 KB
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• 作者单位：Xiaoting Zhang (1) (2)
  Ying Zhao (1)
  Yanting Ye (1)
  Shuifeng Li (1)
  Shiling Qi (1)
  Yuqing Yang (1)
  Hui Cao (1)
  Jian Yang (1)
  Xingqi Zhang (1)
  
  1. Department of Dermatology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510080, Guangdong, People’s Republic of China
  2. Department of Dermatology, The Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, People’s Republic of China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Dermatology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-069X

文摘

Alopecia areata (AA) is a chronic inflammatory disease mediated by an array of cells and cytokines. Immunohistochemistry (IHC) of histological sections with antibodies to mast cell tryptase, CD4, CD8, CD1a and semi-quantitative real-time PCR analysis of Th1- and Th2-type cytokines were performed in 55 patients to investigate the infiltration features of mast cells (MCs), T lymphocytes and Langerhans cells (LCs) in scalp lesions of patients with AA. In AA patients, increased MCs mainly infiltrated the peri-follicular and peri-vascular areas, and correlated positively with numbers of CD8+ T lymphocytes in deep peri-follicular areas (P?=?0.04), but negatively with CD4+ T lymphocytes in deep peri-vascular areas (P?=?0.031). In patients with active hair loss, LCs in epidermis, deep dermis and peri-vascular were elevated (Ps?<?0.05). Infiltration of LCs in upper peri-vascular areas and CD8+ T cell infiltration in deep peri-follicular areas were positively correlated (R?=?0.618, P?=?0.011), as well as LCs in deep peri-vascular areas with CD8+ T cells in upper peri-follicular areas (R?=?0.570, P?=?0.017). In patients with active hair loss, Th1-type cytokine (IL-2, IL-8, TNF-α) mRNA expression in deep dermis were higher than in upper dermis (Ps?<?0.05). However, in patients with non-active hair loss, Th2-type cytokine (IL-5, IL-10) mRNA expression in deep dermis was higher than that in the upper dermis (Ps?<?0.05). Positive correlations were found existing between MCs and CD8+ T cells, as well as between LCs and CD8+ T cells. In conclusion, findings in this study allow us to propose a close relationship between mast cells and CD8+ T cells, as well as between LCs and CD8+ T cells in AA, as well as allergy may interfere with infiltrating T lymphocytes in AA lesional regions. Also, Th1-type cytokine are related to disease activity of alopecia areata, whereas Th2-type cytokines may be associated with persistence of AA.