The extracts of Holotrichia diomphalia larvae inhibit proliferation and induce apoptosis of cancer cells in vitro and in vivo

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• 作者：Lianlian Song (1) (4)
  Shu Sun (2)
  Longnan Jin (3)
  Lijuan Xue (1)
  Yaowen Fu (1)
  
• 关键词：Holotrichia diomphalia ; Grub extracts ; HeLa cells ; Tumor ; Apoptosis ; Proliferation
• 刊名：Molecular & Cellular Toxicology
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：10
• 期：3
• 页码：251-259
• 全文大小：467 KB
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• 作者单位：Lianlian Song (1) (4)
  Shu Sun (2)
  Longnan Jin (3)
  Lijuan Xue (1)
  Yaowen Fu (1)
  
  1. Urology and Nephrology Center, The First Bethune Hospital of Jilin University, Changchun, Jilin, 130021, China
  4. Department of Pathology, Jilin Academy of Traditional Chinese Medicine, Changchun, Jilin, 130021, China
  2. Department of Pathology and Forensic Medicine, Basic Medical College, Yanbian University Health Science Center, Yanbian, Jilin, 133000, China
  3. Jilin Tianping Judicial Appraisal Center, Yanbian, Jilin, 133000, China
  
• ISSN：2092-8467

文摘

Grub extract comes from Holotrichia diomphalia larvae. Numerous studies have demonstrated that grub extracts inhibit the growth of several types of cancer cells in vitro. Here, the anti-tumor activities of grub extracts were observed in experiments performed in vitro and in vivo. Human cervical carcinoma HeLa cells were treated with a range of grub extract doses. Grub extract treatment also resulted in characteristic apoptotic morphological changes in HeLa cells. In vivo acute oral toxicity tests showed that the median lethal dose (LD50sub>) at 5 d was 48.73 g/kg (with 95% confident limits of 46.43-1.14 g/kg) in Kunming mice. Moreover, when the mice were treated with grub extracts at doses of 1.95, 3.9, and 7.8 g/(kg·d) for 10 consecutive days, the solid tumor growth of mice bearing Sl80 was 18.64%, 39.47%, and 53.24%, respectively, relative to the control (P