The growth and reproduction performance of TALEN-mediated β-lactoglobulin-knockout bucks

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• 作者：Hengtao Ge ; Chenchen Cui ; Jun Liu ; Yan Luo ; Fusheng Quan ; Yaping Jin…
• 关键词：Gene targeting ; Reproductive performance ; Recloning ; Goat
• 刊名：Transgenic Research
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：25
• 期：5
• 页码：721-729
• 全文大小：393 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Molecular Medicine
  Plant Genetics and Genomics
  Animal Genetics and Genomics
  Plant Sciences
  Human Genetics
  
• 出版者：Springer Netherlands
• ISSN：1573-9368
• 卷排序：25

文摘

With the technological development of several engineered endonucleases (EENs), such as zinc-finger nucleases, transcription activator-like effector nucleases (TALENs) and CRISPR/Cas9, gene targeting by homologous recombination has been efficiently improved to generate site-specifically genetically modified livestock. However, few studies have been done to investigate the health and fertility of these animals. The purpose of the present study is to investigate if gene targeting events and a recloning procedure would affect the production traits of EEN-mediated gene targeted bucks. TALEN-mediated β-lactoglobulin (BLG) gene mono-allelic knockout (BLG+/−) goats and bi-allelic knockout (BLG−/−) buck produced by using sequential gene targeting combined with recloning in fibroblasts from BLG+/− buck were used to evaluate their health and fertility. Birth weight and postnatal growth of BLG+/− bucks were similar to the wild-type goats. None of the parameters for both fresh and frozen-thawed semen quality were significantly different in BLG+/− or BLG−/− bucks compared to their corresponding comparators. In vitro fertilization (IVF) test revealed that the proportion of IVF oocytes developing to the blastocyst stage was identical among BLG+/−, BLG−/− and wild-type bucks. Conception rates of artificial insemination were respectively 42.3, 38.0 and 42.6 % for frozen-thawed semen from the BLG+/−, BLG−/− and wild-type bucks. In addition, germline transmission of the targeted BLG modification was in accordance with Mendelian rules. These results demonstrated that the analyzed growth and reproductive traits were not impacted by targeting BLG gene and recloning, implicating the potential for dairy goat breeding of BLG+/− and BLG−/− bucks.