Metagenomic characterization of viral communities in Goseong Bay, Korea
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- 作者：Jinik Hwang ; So Yun Park ; Mirye Park ; Sukchan Lee ; Yeonhwa Jo…
- 关键词：marine virus ; viral taxonomy ; metagenomics ; Goseong Bay
- 刊名：Ocean Science Journal
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：51
- 期：4
- 页码：599-612
- 全文大小：
- 刊物类别：Earth and Environmental Science
- 刊物主题：Oceanography; Marine & Freshwater Sciences; Waste Water Technology / Water Pollution Control / Water Management / Aquatic Pollution;
- 出版者：Korea Ocean Research and Development Institute and The Korean Society of Oceanography
- ISSN：2005-7172
- 卷排序：51
文摘
In this study, seawater samples were collected from Goseong Bay, Korea in March 2014 and viral populations were examined by metagenomics assembly. Enrichment of marine viral particles using FeCl3 followed by next-generation sequencing produced numerous sequences. De novo assembly and BLAST search showed that most of the obtained contigs were unknown sequences and only 0.74% of sequences were associated with known viruses. As a result, 138 viruses, including bacteriophages (87%), viruses infecting algae and others (13%) were identified. The identified 138 viruses were divided into 11 orders, 14 families, 34 genera, and 133 species. The dominant viruses were Pelagibacter phage HTVC010P and Roseobacter phage SIO1. The viruses infecting algae, including the Ostreococcus species, accounted for 9.4% of total identified viruses. In addition, we identified pathogenic herpes viruses infecting fishes and giant viruses infecting parasitic acanthamoeba species. This is a comprehensive study to reveal the viral populations in the Goseong Bay using metagenomics. The information associated with the marine viral community in Goseong Bay, Korea will be useful for comparative analysis in other marine viral communities.