Arabidopsis AtERF71/HRE2 functions as transcriptional activator via cis-acting GCC box or DRE/CRT element and is involved in root development through regulation of root cell expansion

详细信息    查看全文

• 作者：Sun-Young Lee ; Eun Young Hwang ; Hye-Yeon Seok ; Vaishali N. Tarte…
• 关键词：AtERF71/HRE2 ; DRE/CRT ; GCC box ; Transactivation
• 刊名：Plant Cell Reports
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：34
• 期：2
• 页码：223-231
• 全文大小：1,050 KB
• 参考文献：1. Allen MD, Yamasaki K, Ohme-Takagi M, Tateno M, Suzuki M (1998) A novel mode of DNA recognition by a β-sheet revealed by the solution structure of the GCC-box binding domain in complex with DNA. EMBO J 17:5484-496 CrossRef
  2. Beemster GT, Baskin TI (1998) Analysis of cell division and elongation underlying the developmental acceleration of root growth in / Arabidopsis thaliana. Plant Physiol 116:1515-526 CrossRef
  3. Hao DY, Ohme-Takagi M, Sarai A (1998) Unique mode of GCC box recognition by the DNA-binding domain of ethylene-responsive element-binding factor (ERF domain) in plant. J Biol Chem 273:26857-6861 CrossRef
  4. Hess N, Klode M, Andres M, Sauter M (2011) The hypoxia responsive transcription factor genes ERF71/HRE2 and ERF73/HRE1 of / Arabidopsis are differentially regulated by ethylene. Physiol Plant 143:41-9 CrossRef
  5. Lee JH, Kim DM, Lee JH, Kim J, Bang JW, Kim WT, Pai HS (2005) Functional characterization of NtCEF1, an AP2/EREBP-type transcriptional activator highly expressed in tobacco callus. Planta 222:211-24 CrossRef
  6. Licausi F, van Dongen JT, Giuntoli B, Novi G, Santaniello A, Geigenberger P, Perata P (2010) HRE1 and HRE2, two hypoxia-inducible ethylene response factors, affect anaerobic responses in / Arabidopsis thaliana. Plant J 62:302-15 CrossRef
  7. Okamuro JK, Caster B, Villarroel R, Montagu MV, Jofuku KD (1997) The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in / Arabidopsis. Proc Natl Acad Sci USA 94:7076-081 CrossRef
  8. Park JM, Park CJ, Park SB, Ham BK, Shin R, Paek KH (2001) Overexpression of the tobacco Tsi1 gene encoding an EREBP/AP2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco. Plant Cell 13:1035-046 CrossRef
  9. Park HY, Lee SY, Seok HY, Kim SH, Sung ZR, Moon YH (2011a) EMF1 interacts with EIP1, EIP6 or EIP9 involved in the regulation of flowering time in / Arabidopsis. Plant Cell Physiol 52:1376-388 CrossRef
  10. Park HY, Seok HY, Woo DH, Lee SY, Tarte VN, Lee EH, Lee CH, Moon YH (2011b) AtERF71/HRE2 transcription factor mediates osmotic stress response as well as hypoxia response in / Arabidopsis. Biochem Biophys Res Commun 414:135-41 CrossRef
  11. Sakuma Y, Liu Q, Dubouzet JG, Abe H, Shinozaki K, Yamaguchi-Shinozaki K (2002) DNA-binding specificity of the ERF/AP2 domain of / Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression. Biochem Biophys Res Commun 290:998-009 CrossRef
  12. Seok HY, Tarte VN, Lee SY, Park HY, Moon YH (2014) / Arabidopsis HRE1α, a splicing variant of AtERF73/HRE1, functions as a nuclear transcription activator in hypoxia response and root development. Plant Cell Rep 33:1255-262 CrossRef
  13. Sheen J (2001) Signal transduction in maize and / Arabidopsis mesophyll protoplasts. Plant Physiol 127:1466-475 CrossRef
  14. Tang M, Sun J, Liu Y, Chen F, Shen S (2007) Isolation and functional characterization of the JcERF gene, a putative AP2/EREBP domain-containing transcription factor, in the woody oil plant / Jatropha curcas. Plant Mol Biol 63:419-28 CrossRef
  15. Thomashow MF (1999) Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu Rev Plant Physiol Plant Mol
• 作者单位：Sun-Young Lee (1)
  Eun Young Hwang (1)
  Hye-Yeon Seok (1)
  Vaishali N. Tarte (1)
  Mi Suk Jeong (1)
  Se Bok Jang (1)
  Yong-Hwan Moon (1)
  
  1. Department of Molecular Biology, Pusan National University, Busan, 609-735, Korea
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Biotechnology
  Plant Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-203X

文摘

Key message AtERF71/HRE2 binds to GCC box or DRE/CRT as transcription activator and plays an important role in root development via root cell expansion regulation. Abstract AtERF71/HRE2 transcription factor, a member of the AP2/ERF family, plays a key role in the stress response. GCC box and DRE/CRT, both essential cis-acting elements, have been shown to be recognized by AP2/ERF family transcription factors. However, it remains unclear whether or not AtERF71/HRE2 directly interacts with GCC box and/or DRE/CRT. Here, we showed that AtERF71/HRE2 binds to GCC box and DRE/CRT by electrophoretic mobility shift assay (EMSA). Binding of AtERF71/HRE2 to GCC box and DRE/CRT was also detected by fluorescence measurement and surface plasmon resonance spectroscopy (BIAcore) experiments. Folding properties of AtERF71/HRE2 proteins were characterized by CD spectroscopy, and AtERF71/HRE2 showed thermal stability as evidenced by two endothermic peaks (T d) at 53 and 65?°C. In addition, AtERF71/HRE2 showed transcriptional activation activity via GCC box and DRE/CRT in Arabidopsis protoplasts. Interestingly, AtERF71/HRE2 OXs showed increased primary root length due to elevated root cell expansion. Our data indicate that AtERF71/HRE2 binds to both GCC box and DRE/CRT, transactivates expression of genes downstream via GCC box or DRE/CRT, and plays an important role in root development through regulation of root cell expansion.