Identification of quantitative trait loci responsible for rice grain protein content using chromosome segment substitution lines and fine mapping of qPC-1 in rice (Oryza sativa L.)

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• 作者：Yihao Yang ; Min Guo ; Rongde Li ; Lan Shen ; Wei Wang ; Min Liu ; Qian Zhu…
• 关键词：Rice ; Protein content ; qPC ; 1 ; Fine mapping
• 刊名：Molecular Breeding
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：35
• 期：6
• 全文大小：566 KB
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• 作者单位：Yihao Yang (1)
  Min Guo (1)
  Rongde Li (1)
  Lan Shen (1)
  Wei Wang (1)
  Min Liu (1)
  Qian Zhu (1)
  Zhi Hu (1)
  Qiangwei He (1)
  Yang Xue (1)
  Shuzhu Tang (1)
  Minghong Gu (1)
  Changjie Yan (1)
  
  1. Jiangsu Key Laboratory of Crop Genetics and Physiology, Co-Innovation Center for Modern Production Technology of Grain Crops, Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture, Yangzhou University, Yangzhou, 225009, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-9788

文摘

Protein content (PC) in rice endosperm plays an important role in determining rice grain quality. However, the genetic mechanism underlying grain PC remains unclear. In order to better understand the genetic basis of this trait, a chromosome segment substitution line (CSSL) population derived from the cross of Sasanishiki/Habataki was employed for genetic analysis and gene mapping. In three environments, seven quantitative trait loci in total were identified, of which only qPC-1 was repeatedly detected across three environments, and qPC-10 was identified in two environments; the other five QTLs were detected in one environment. In order to fine-map qPC-1, a CSSL with low PC, SL402, harboring qPC-1, was crossed with Sasanishiki to develop F₂ and F₃ segregation populations. qPC-1 was finally delimited to a 41-kb DNA region on chromosome 1. Storage protein component analysis indicated that the allele from Habataki on qPC-1 can significantly decrease the glutelin content, consequently leading to the decrease in PC. These results provide an important aid for map-based cloning of qPC-1, and the markers linked to qPC-1 could be applied to rice quality improvement.