Novel compounds that enhance Agrobacterium-mediated plant transformation by mitigating oxidative stress
详细信息 查看全文
- 作者：Yinghui Dan ; Song Zhang ; Heng Zhong ; Hochul Yi ; Manuel B. Sainz
- 关键词：Agrobacterium tumefaciens ; Anti ; oxidative compounds ; Cell death ; Oxidative stress ; Plant transformation ; Tissue browning
- 刊名：Plant Cell Reports
- 出版年：2015
- 出版时间：February 2015
- 年：2015
- 卷：34
- 期：2
- 页码：291-309
- 全文大小：2,184 KB
- 参考文献：1. Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373-99 CrossRef
2. Arnao MB, Hernandez-Ruiz A (2007) Melatonin promotes adventitious- and lateral root regeneration in etiolated hypocotyls of / Lupinus albus L. J Pineal Res 42:147-52 CrossRef
3. Barampuram S, Zhang JZ (2011) Recent Advances in Plant Transformation. Plant chromosome engineering: methods and protocols, methods in molecular biology. Springer, New York, pp 1-5 CrossRef
4. Chen S, Dickman MB (2004) Bcl-family members localize to tobacco chloroplasts and inhibit programmed cell death induced by chloroplast-targeted herbicides. J Exp Bot 8:1-
5. Chen Z, Silva H, Klessig DF (1993) Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. Science 262:1883-886 CrossRef
6. Chen Q, Qi WB, Reiter RJ, Wei W, Wang BM (2009) Exogenously applied melatonin stimulates root growth and raises endogenous indoleacetic acid in roots of etiolated seedlings of / Brassica juncea. J Plant Physiol 166:324-28 CrossRef
7. Crescioli C, Cosmi L, Borgogni E, Santarlasci V, Gelmini S, Sottili M, Sarchielli E, Mazzinghi B, Francalanci M, Pezzatini A, Perigli G, Vanneli GB, Annunziato F, Serio M (2007) Methimazole inhibits CXC chemokine ligand 10 secretion in human thyrocytes. J Endocrinol 195:145-55 CrossRef
8. Dan Y (2008) Biological functions of antioxidants in plant transformation. In Vitro Cell Dev Biol Plant 44:149-61 CrossRef
9. Dan Y, Munyikawa TRI, Kimberly AR, Rommens CMT (2004) Use of lipoic acid in plant culture media. US Patent Application US 2004/0133938 A1
10. Dan Y, Yan H, Munyikwa T, Dong J, Zhang Y, Armstrong CL (2006) MicroTom—a high-throughput model transformation system for functional genomics. Plant Cell Rep 25:432-41 CrossRef
11. Dan Y, Armstrong CL, Dong J, Feng X, Fry JE, Keithly GE, Martinell BJ, Roberts GA, Smith LA, Tan LJ, Duncan DR (2009) Lipoic acid—a unique plant transformation enhancer. In Vitro Cell Dev Biol Plant 45:630-38 CrossRef
12. Dan Y, Sainz MB, Zhong H, Shi L (2013) Compositions and methods for modulating oxidative burst in plant transformation. US Patent No. US8481319B2
13. Das D, Reddy M, Upadhyaya K, Sopory S (2002) An efficient leaf-disc culture method for the regeneration via somatic embryogenesis and transformation of grape ( / Vitis vinifera L.). Plant Cell Rep 20:999-005 CrossRef
14. De Tullio MC, Jiang K, Feldman LJ (2010) Redox regulation of root apical meristem organization: connecting root development to its environment. Plant Physiol Biochem 48:328-36 CrossRef
15. Deneke SM (2000) Thiol-based antioxidants. In: Stadtman ER, Chock PB (eds) Current topics in cellular regulation. Academic Press, San Diego, pp 151-80
16. Dickman MB, Park VK, Oltersdorf T, Li W, Clemente T, French R (2001) Abrogation of disease development in plants expressing animal anti-apoptotic genes. Proc Natl Acad Sci USA 98:6957-962 CrossRef
17. Dobocovich ML, Markowska M (2005) Functional MT1 and MT2 melatonin receptors in mammals. Endocrinology 27:101-10
18. Doke N (1983) Involvement of superoxide anion generation in hypersensitive response of potato tuber tissues to infection with an incompatible race of / Phytophthora infestans. Physiol Plant Pathol 23:345-47 Yinghui Dan (1) (2) (3)
Song Zhang (1)
Heng Zhong (4)
Hochul Yi (4)
Manuel B. Sainz (4)
1. Institute for Advanced Learning and Research, 150 Slayton Avenue, Danville, VA, 24540, USA
2. Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA
3. Department of Forest Resources and Environmental Conservation, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA
4. Syngenta Biotechnology, Inc., 3054 East Cornwallis Road, Research Triangle Park, NC, 27709, USA - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Cell Biology
Plant Sciences
Biotechnology
Plant Biochemistry - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-203X
文摘
Key message Agrobacterium tumefaciens caused tissue browning leading to subsequent cell death in plant transformation and novel anti-oxidative compounds enhanced Agrobacterium -mediated plant transformation by mitigating oxidative stress. Abstract Browning and death of cells transformed with Agrobacterium tumefaciens is a long-standing and high impact problem in plant transformation and the agricultural biotechnology industry, severely limiting the production of transgenic plants. Using our tomato cv. MicroTom transformation system, we demonstrated that Agrobacterium caused tissue browning (TB) leading to subsequent cell death by our correlation study. Without an antioxidant (lipoic acid, LA) TB was severe and associated with high levels of GUS transient expression and low stable transformation frequency (STF). LA addition shifted the curve in that most TB was intermediate and associated with the highest levels of GUS transient expression and STF. We evaluated 18 novel anti-oxidative compounds for their potential to enhance Agrobacterium-mediated transformation, by screening for TB reduction and monitoring GUS transient expression. Promising compounds were further evaluated for their effect on MicroTom and soybean STF. Among twelve non-antioxidant compounds, seven and five significantly (P?Agrobacterium-mediated TB and increase STF, strongly supporting that oxidative stress is an important limiting factor in Agrobacterium-mediated transformation and the limiting factor can be controlled by these compounds at different levels.