Development of an efficient transformation method by Agrobacterium tumefaciens and high throughput spray assay to identify transgenic plants for woodland strawberry (Fragaria vesca) using NPTII se
详细信息 查看全文
- 作者：Christopher J. Pantazis (1)
Sarah Fisk (1)
Kerri Mills (2)
Barry S. Flinn (1) (2) (3)
Vladimir Shulaev (4)
Richard E. Veilleux (2)
Yinghui Dan (1) (2) (3) - 关键词：Agrobacterium tumefaciens ; mediated transformation ; Woodland strawberry ; Kanamycin selection ; Paromomycin spray assay
- 刊名：Plant Cell Reports
- 出版年：2013
- 出版时间：March 2013
- 年：2013
- 卷：32
- 期：3
- 页码：329-337
- 全文大小：564KB
- 参考文献：1. Abdal-Aziz SA, Pliego-Alfaro F, Quesada MA, Mercado JA (2006) Evidence of frequent integration of non-T-DNA vector backbone sequences in transgenic strawberry plant. J Biosci Bioeng 101:508-10 CrossRef
2. Ayliffe MA, Pallotta M, Langridge P, Pryor AJ (2007) A barley activation tagging system. Plant Mol Biol 64:329-47 CrossRef
3. Cervera M, Juarez J, Navarro A, Pina JA, Duran-Vila N, Navarro L, Pena L (1998) Genetic transformation and regeneration of mature tissue of woody fruit plants bypassing the juvenile stage. Transgenic Res 7:51-9 CrossRef
4. Cheng M, Fry JE, Pang S, Zhou H, Hironaka CM, Duncan Dr, Conner TW, Wan Y (1997) Genetic transformation of wheat mediated by / Agrobacterium tumefaciens. Plant Physiol 115:971-80
5. Cheng M, Lowe BA, Spencer MT, Ye X, Armstrong CL (2004) Factors influencing / Agrobacterium-mediated transformation of monocotyledonous species. In Vitro Cell Dev Biol Plant 40:31-5 CrossRef
6. Cordero de Mesa M, Jimnez-Bermudez S, Pliego-Alfaro F, Quesada MA, Mercado JA (2000) / Agrobacterium cells as microprojectile coating: a novel approach to enhance stable transformation rates in strawberry. Aust J Plant Physiol 27:1093-100
7. Cordero de Mesa M, Santiago-Doménech N, Pliego-Alfaro F, Quesada MA, Mercado JA (2004) The CaMV 35S promoter is highly active on floral and pollen of transgenic strawberry plants. Plant Cell Rep 23:32-8
8. Folta KM, Dhingra A (2006) Transformation of strawberry: the basis for translational genomics in rosaceae. In Vitro Cell Dev Biol Plant 42:482-90 CrossRef
9. Ipek A, Masson P, Simon PW (2006) Genetic transformation of an / Ac/Ds-based transposon tagging system in carrot ( / Daucus carota L.). Eur J Hort Sci 71:245-51
10. James DJ, Passey AJ, Barbara DJ, Bevan M (1989) Genetic transformation of apple ( / Malus pumila Mill) using a disarmed Tibinary vector. Plant Cell Rep 7:658-61
11. Kolesnik T, Szeverenyi I, Bachmann D, Kumar CS, Jiang S, Ramamoorthy R, Cai M, Ma ZG, Sundaresan V, Ramachandran S (2004) Establishing an efficient / Ac/Ds tagging system in rice: large-scale analysis of / Ds flanking sequences. Plant J 37:301-14 CrossRef
12. Kuta DD, Tripathi L (2005) / Agrobacterium-induced hypersensitive necrotic reaction in plant cells: a resistance response against / Agrobacterium-mediated DNA transfer. Afr J Biotechnol 4(8):752-57
13. Moore GA, Jacono CC, Neidigh JL, Lawrence SD, Cline K (1992) / Agrobacterium-mediated transformation of citrus stem segments and regeneration of transgenic plants. Plant Cell Rep 11:238-42 CrossRef
14. Mourgues F, Chevreau E, Lambert C, Bondt A (1996) Efficient / Agrobacterium-mediated transformation and recovery of transgenic plants from pear ( / Pyrus communis L.). Plant Cell Rep 16:245-49
15. Murkute A, Patil A, Mayakumari S (2003) Exudation and browning in tissue culture of pomegranate. Agric Sci Dig 23(1):29-1
16. Oosumi T, Gruszewski HA, Blischak LA, Baxter AJ, Wadl PA, Shuman JL, Veilleux RE, Shulaev V (2006) High-efficiency transformation of the diploid strawberry ( / Fragaria vesca) for functional genomics. Planta 223:1219-230 CrossRef
17. Oosumi T, Ruiz-Rojas JJ, Veilleux RE, Dickerman A, Shulaev V (2010) Implementing reverse genetics in Rosaceae: analysis of T-DNA flanking sequences of insertional mutant lines in the diploid strawberry, Fragaria vesca. Physiol Plant 140:1- CrossRef
18. Pastori GM, Huttly A, West J, Sparks C, Pieters A, Luna CM, Jones HD, Foyer CH (2007) The maize activator/dissociation system is functional in hexaploid wheat through successive generations. Func Plant Biol 34:835-43 CrossRef
19. Pena L, Cervera M, Juarez J, Ortega C, Pina JA, Duran-Vila N, Navarro L (1995a) High-efficiency / Agrobacterium-mediated transformation and regeneration of citrus. Plant Sci 104:183-91 CrossRef
20. Pena L, Cervera M, Juarez J, Navarro A, Pina JA, Duran-Vila N, Navarro L (1995b) / Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants. Plant Cell Rep 14:616-19 CrossRef
21. Pena L, Cervera M, Juarez J, Navarro A, Pina JA, Navarro L, Rep PC (1997) Genetic transformation of lime ( / Citrus aurantifolia Swing.): factors affecting transformation and regeneration. Plant Cell Rep 16:731-37 CrossRef
22. Perl A, Lotan O, Abu-Abied M, Holland D (1996) Establishment of an / Agrobacterium-mediated transformation system for grape ( / Vitis vinifera L.): establishment of an / Agrobacterium-mediated transformation system for grape ( / Vitis vinifera L.): the role of antioxidants during grape- / Agrobacterium interactions. Nat Biotechnol 14(5):624-28 CrossRef
23. Qin Y, Teixeira da Silva JA, Zhang L, Zhang S (2008) Transgenic strawberry: state of the art for improved traits. Biotechnol Adv 26:219-32 CrossRef
24. Qin YH, Teixeira da Silva JA, Bi JH, Zhang SL, Hu GB (2011) Response of in vitro strawberry to antibiotics. Plant Growth Regul 65:183-93 CrossRef
25. Sheikholeslam SN, Weeks DP (1987) Acetosyringone promotes high efficiency transformation of / Arabidopsis thaliana explants by / Agrobacterium tumefaciens. Plant Mol Bio 8:291-98 CrossRef
26. Shulaev V, Sargent DJ, Crowhurst RN et al (2011) The genome of woodland strawberry ( / Fragaria vesca). Nat Genet 43:109-16 CrossRef
27. Stipic M, Rotino GL, Piro F (2000) Regeneration and genetic transformation attempts in the cauliflower ‘Tardivo di Fano- Italus Hortus 7(2):20-6
28. Veilleux RE, Mills KP, Baxter AJ, Upham KT, Ferguson TJ, Holt SH, Lu N, Ruiz-Rojas JJ, Pantazis C, Davis CM, Lindsey RC, Powell FL, Dan Y, Dickerman A, Oosumi T, Shulaev V (2012) Transposon tagging in diploid strawberry. Plant Biotechnol J 10:985-94 CrossRef
29. Woody ST, Austin-Phillips S, Amasino RM, Krysan PJ (2007) The WiscDsLox T-DNA collection: an arabidopsis community resource generated by using an improved high-throughput T-DNA sequencing pipeline. J Plant Res 120:157-65 CrossRef
30. Wu H, Sparks C, Amoah B, Jones HD (2003) Factors influencing successful / Agrobacterium-mediated genetic transformation of wheat. Plant Cell Rep 21:659-68
31. Zale JM, Agarwal S, Loar S, Steber CM (2009) Evidence for stable transformation of wheat by floral dip in / Agrobacterium / tumefaciens. Plant Cell Rep 28:903-13 CrossRef
32. Zhang HM, Wang JL (2005) Establishment of genetic transformation system of “Allstar-strawberry leaf. Biotechnology 15:68-0
33. Zhao Y, Liu Q, Davis RE (2004) Transgene expression in strawberries driven by a heterologous phloem-specific promoter. Plant Cell Rep 23:224-30 CrossRef - 作者单位：Christopher J. Pantazis (1)
Sarah Fisk (1)
Kerri Mills (2)
Barry S. Flinn (1) (2) (3)
Vladimir Shulaev (4)
Richard E. Veilleux (2)
Yinghui Dan (1) (2) (3)
1. Institute for Advanced Learning and Research, 150 Slayton Avenue Danville, Danville, VA, 24540, USA
2. Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA
3. Department of Forest Resources and Environmental Conservation, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA
4. Department of Biological Sciences, University of North Texas, Denton, TX, USA - ISSN：1432-203X
文摘
Key message We developed an efficient Agrobacterium -mediated transformation method using an Ac/Ds transposon tagging construct for F. vesca and high throughput paromomycin spray assay to identify its transformants for strawberry functional genomics. Abstract Genomic resources for Rosaceae species are now readily available, including the Fragaria vesca genome, EST sequences, markers, linkage maps, and physical maps. The Rosaceae Genomic Executive Committee has promoted strawberry as a translational genomics model due to its unique biological features and transformability for fruit trait improvement. Our overall research goal is to use functional genomic and metabolic approaches to pursue high throughput gene discovery in the diploid woodland strawberry. F. vesca offers several advantages of a fleshy fruit typical of most fruit crops, short life cycle (seed to seed in 12-6?weeks), small genome size (206?Mbb/C), small plant size, self-compatibility, and many seeds per plant. We have developed an efficient Agrobacterium tumefaciens-mediated strawberry transformation method using kanamycin selection, and high throughput paromomycin spray assay to efficiently identify transgenic strawberry plants. Using our kanamycin transformation method, we were able to produce up to 98 independent kanamycin resistant insertional mutant lines using a T-DNA construct carrying an Ac/Ds transposon Launchpad system from a single transformation experiment involving inoculation of 22 leaf explants of F. vesca accession 551572 within approx. 11?weeks (from inoculation to soil). Transgenic plants with 1- copies of a transgene were confirmed by Southern blot analysis. Using our paromomycin spray assay, transgenic F. vesca plants were rapidly identified within 10?days after spraying.