Comparative expression analysis of three genes from the Arabidopsis vacuolar Na+/H+ antiporter (AtNHX) family in relation to abiotic stresses
详细信息 查看全文
- 作者：WeiQuan Wang (1)
Yin Li (1)
YiYue Zhang (1) (2)
CuiPing Yang (1)
NuoYan Zheng (1) (2)
Qi Xie (1) (2) - 关键词：NHX antiporter ; abiotic stress ; expression pattern ; salt tolerance
- 刊名：Chinese Science Bulletin
- 出版年：2007
- 出版时间：July 2007
- 年：2007
- 卷：52
- 期：13
- 页码：1754-1763
- 全文大小：6736KB
- 参考文献：1. Blumwald E G, Aharon S, Apse M P. Sodium transport in plant cells. Biochim Biophys Acta, 2000, 1465: 140-51 CrossRef
2. Hasegawa P M, Bressan R A, Zhu J K, et al. Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol, 2000, 51: 463-99 CrossRef
3. Niu X, Bressan R A, Hasegawa P M, et al. Ion homeostasis in NaCl stress environments. Plant Physiol, 1995, 109: 735-42
4. Apse M P, Aharon G S, Snedden W A, et al. Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in / Arabidopsis. Science, 1999, 285: 1256-258 CrossRef
5. Fukuda A, Nakamura A, Tanaka Y. Molecular cloning and expression of the Na+/H+ exchanger gene in / Oryza sativa. Biochim Biophys Acta, 1999, 1446: 149-55
6. Chauhan S, Forsthoefel N, Ran Y, et al. Na+/myo-inositol symporters and Na+/H+-antiport in / Mesembryanthemum crystallinum. Plant J, 2000, 24: 511-22 CrossRef
7. Hamada A, Shono M, Xia T, et al. Isolation and characterization of a Na+/H+ antiporter gene from the halophyte / Atriplex gmelini. Plant Mol Biol, 2001, 46: 35-2 CrossRef
8. Tao X, Maris P A, Gilad S A, et al. Identification and characterization of a NaCl-inducible vacuolar Na+/H+ antiporter in / Beta vulgaris. Physiol Plant, 2002, 116: 206-12 CrossRef
9. Wang J, Zuo K J, Tang K X. Molecular cloning and characterization of a new Na+/H+ antiporter gene from / Brassica napus. DNA Sequence, 2003, 14: 351-58
10. Maser P, Thomine S, Schroeder J I, et al. Phylogenetic relationships within cation transporter families of / Arabidopsis. Plant Physiol, 2001, 126: 1646-667 CrossRef
11. Aharon G S, Apse M P, Duan S L, et al. Characterization of a family of vacuolar Na+/H+ antiporters in / Arabidopsis thaliana. Plant Soil, 2003, 253: 245-56 CrossRef
12. Apse M P, Sottosanto J B, Blumwald E. Vacuolar cation/H+ exchange, ion homeostasis, and leaf development are altered in a T-DNA insertional mutant of / AtNHX1, the / Arabidopsis vacuolar Na+/H+ antiporter. Plant J, 2003, 36: 229-39 CrossRef
13. Yokoi S, Quintero F J, Cubero B, et al. Differential expression and function of / Arabidopsis thaliana NHX Na+/H+ antiporters in the salt stress response. Plant J, 2002, 30: 529-39 CrossRef
14. Shi H, Lee B H, Wu S J, et al. Overexpression of a plasma membrane Na+/H+ antiporter gene improves salt tolerance in / Arabidopsis thaliana. Nat Biotechnol, 2003, 21: 81-5 CrossRef
15. Ohta M, Hayashi Y, Nakashima A, et al. Introduction of a Na+/H+ antiporter gene from / Atriplex gmelini confers salt tolerance to rice. FEBS Lett, 2002, 532: 279-82 CrossRef
16. Fukuda A, Nakamura A, Tagiri A, et al. Function, intracellular localization and the importance in salt tolerance of a vacuolar Na+/H+ antiporter from rice. Plant Cell Physiol, 2004, 45: 146-59 CrossRef
17. Zhang H X, Hodson J N, Williams J P, et al. Engineering salt-tolerant / Brassica plants: Characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation. Proc Natl Acad Sci USA, 2001, 98: 12832-2836 CrossRef
18. Zhang H X, Blumwald E. Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nat Biotechnol, 2001, 19: 765-68 CrossRef
19. Yamaguchi T, Fukada-Tanaka S, Inagaki Y, et al. Genes encoding the vacuolar Na+/H+ exchanger and flower coloration. Plant Cell Physiol, 2001, 42: 451-61 CrossRef
20. Shi H, Zhu J K. Regulation of expression of the vacuolar Na+/H+ antiporter gene / AtNHX1 by salt stress and abscisic acid. Plant Mol Biol, 2002, 50: 543-50 CrossRef
21. Brett C L, Donowitz M, Rao R. Evolutionary origins of eukaryotic sodium/proton exchangers. Am J Physiol Cell Physiol, 2005, 288: C223–C239 CrossRef
22. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant, 1962, 15: 473-97 CrossRef
23. Chang S S, Park S K, Kim B C, et al. Stable genetic transformation of / Arabidopsis thaliana by / Agrobacterium inoculation in planta. Plant J, 1994, 5: 551-58
24. Bechtold N, Ellis J, Pellertier G. In Planta agrobacterium mediated gene transfer by infiltration of adult / Arabidopsis thaliana plants. C R Acad Sci Paris Life Sci, 1993, 316: 1194-199
25. Liu Y G, Mitsukawa N, Oosumi T, et al. Efficient isolation and mapping of / Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. Plant J, 1995, 8: 457-63 CrossRef
26. Sambrook J, Fritsch E F, Maniatis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989
27. Jefferson R A. Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol Biol Rep, 1987, 5: 387-05 CrossRef
28. Thompson J D, Gibson T J, Plewniak F, et al. The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 1997, 25: 4876-882 CrossRef
29. Wilkinson M. Majority-rule reduced consensus trees and their use in bootstrapping. Mol Biol Evol, 1996, 13: 437-44
30. Swofford D L. PAUP*: Phylogenetic analysis using parsimony (* and other methods). version 4.0b1, Sinauer Associates, 1998
31. Zimmermann P, Hirsch-Hoffmann M, Henning L, et al. GENEVESTIGATOR / Arabidopsis microarray database and analysis toolbox. Plant Physiol, 2004, 136: 2621-632 CrossRef
32. Hughes A L. The evolution of functionally novel proteins after gene duplication. Proc Biol Sci, 1994, 256: 119-24 CrossRef
33. Shinozaki K, Yamaguchi-Shinozaki K. Molecular responses to dehydration and low temperature: Differences and cross-talk between two stress signaling pathways. Curr Opin Plant Biol, 2000, 3: 217-23
34. Scholz-Starke J, Buttner M, Sauer N. AtSTP6, a new pollen-specific H+-monosaccharide symporter from / Arabidopsis. Plant Physiol, 2003, 131: 70-7 CrossRef
35. Schneidereit A, Scholz-Starke J, Buttner M. Functional characterization and expression analyses of the glucose-specific / AtSTP9 monosaccharide transporter in pollen of / Arabidopsis. Plant Physiol, 2003, 133: 182-90 CrossRef
36. Lee Y H, Tegeder M. Selective expression of a novel high-affinity transport system for acidic and neutral amino acids in the tapetum cells of / Arabidopsis flowers. Plant J, 2004, 40: 60-4 CrossRef
37. Venema K, Quintero F J, Pardo J M, et al. The / Arabidopsis Na+/H+ exchanger AtNHX1 catalyzes low affinity Na+ and K+ transport in reconstituted liposomes. J Biol Chem, 2002, 277: 2413-418 CrossRef
38. Sze H, Padmanaban S, Cellier F, et al. Expression patterns of a novel / AtCHX gene family highlight potential roles in osmotic adjustment and K+ homeostasis in pollen development. Plant Physiol, 2004, 136: 2532-547 CrossRef
39. Tester M, Davenport R. Na+ tolerance and Na+ transport in higher plants. Ann Bot, 2003, 91: 503-27 CrossRef
40. Wegner L H, De Boer A H. Properties of two outward-rectifying channels in root xylem parenchyma cells suggest a role in K+ homeostasis and long-distance signaling. Plant Physiol, 1997, 115: 1707-719 - 作者单位：WeiQuan Wang (1)
Yin Li (1)
YiYue Zhang (1) (2)
CuiPing Yang (1)
NuoYan Zheng (1) (2)
Qi Xie (1) (2)
1. State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China
2. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China - ISSN：1861-9541
文摘
Na+/H+ antiporters (NHX) are ubiquitous transmembrane proteins that play a key role in salt tolerance of plants. In this study, the sequence of 3 Arabidopsis NHX genes (AtNHX2-) were compared with other AtNHX members. Putative cis-elements analysis identified elements that have been associated with stress responses. The activities of the promoters AtNHX2- were studied in transgenic plants carrying corresponding promoter-β-glucuronidase (GUS) fusions. The AtNHX2 promoter-GUS analysis indicated that AtNHX2 was expressed in constitutive pattern with high GUS activity in roots and leaves. AtNHX2 promoter activity was not up-regulated by NaCl or abscisic acid (ABA), in contrast the AtNHX1 promoter which was previously studied. The AtNHX3 and AtNHX4 promoters showed tissue-specific activities. Strong GUS activity was detected in roots and vascular bundles of the stele in plants carrying an AtNHX4 promoter-GUS fusion, and GUS activity increased under salt stress suggesting a function related to salt tolerance. Transgenic plants carrying the AtNHX3 promoter-GUS fusion showed strong GUS activity in petals, stamens and tops of siliques, suggesting a possible role of AtNHX3 in flower and seed development. Results of histochemical analysis suggested that AtNHX2- are involved in divergent functions and are differentially regulated under abiotic stress. The structure of AtNHX4 was predicted to include 12 transmembrane regions and a NHX domain. Overexpression of AtNHX4 in Arabidopsis transgenic lines confers greater salt tolerance than in wild type plants. These results suggest that AtNHX4 may encode a putative vacuolar NHX that plays an important role in salt tolerance.