Expression of glycine receptor alpha 3 in the rat trigeminal neurons and central boutons in the brainstem

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• 作者：Jin Young Bae ; Won Mah ; Jong-Cheol Rah ; Sook Kyung Park…
• 关键词：Glycine receptor ; Extrasynaptic ; Primary sensory afferent ; Trigeminal ; Presynaptic modulation ; Ultrastructure
• 刊名：Brain Structure and Function
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：221
• 期：9
• 页码：4601-4613
• 全文大小：3,642 KB
• 刊物主题：Neurosciences; Cell Biology; Neurology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1863-2661
• 卷排序：221

文摘

Increasing evidence shows that the homomeric glycine receptor is expressed in axon terminals and is involved in the presynaptic modulation of transmitter release. However, little is known about the expression of the glycine receptor, implicated in the presynaptic modulation of sensory transmission in the primary somatosensory neurons and their central boutons. To address this, we investigated the expression of glycine receptor subunit alpha 3 (GlyRα3) in the neurons in the trigeminal ganglion and axon terminals in the 1st relay nucleus of the brainstem by light- and electron-microscopic immunohistochemistry. Trigeminal primary sensory neurons were GlyRα3-immunopositive/gephyrin-immunonegative (indicating homomeric GlyR), whereas GlyRα3/gephyrin immunoreactivity (indicating heteromeric GlyR) was observed in dendrites. GlyRα3 immunoreactivity was also found in the central boutons of primary afferents but far from the presynaptic site and in dendrites at subsynaptic sites. Boutons expressing GlyRα3 contained small round vesicles, formed asymmetric synapses with dendrites and were immunoreactive for glutamate. These findings suggest that trigeminal primary afferent boutons receive presynaptic modulation via homomeric, extrasynaptic GlyRα3, and that different subtypes of GlyR may be involved in pre- and postsynaptic inhibition.