Allogeneic Adipose-Derived Stem Cells Protect Fat Grafts at the Early Stage and Improve Long-Term Retention in Immunocompetent Rats
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- 作者：Jun Zhang ; Yunchuan Wang ; Bin Zhao ; Lei Fan ; Xiaozhi Bai…
- 关键词：Adipose ; derived stem cells ; Fat grafts ; Cells transplantation ; Immune rejection
- 刊名：Aesthetic Plastic Surgery
- 出版年：2015
- 出版时间：August 2015
- 年：2015
- 卷：39
- 期：4
- 页码：625-634
- 全文大小：4,536 KB
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Yunchuan Wang (3)
Bin Zhao (3)
Lei Fan (3)
Xiaozhi Bai (3)
Longlong Yang (3)
Peng Chang (2)
Dahai Hu (3)
Xiaoyan Liu (2)
1. Graduate School, Second Military Medical University, Shanghai, 200433, People’s Republic of China
2. Department of Plastic Surgery, General Hospital of Shenyang Military Area Command of PLA, 83 Wenhua Road, Shenyang, 110016, Liaoning, People’s Republic of China
3. Department of Burns and Cutaneous Surgery, Xijing Hospital, Fourth Military Medical University, 127 Changle West Road, Xi’an, 710032, Shaanxi, People’s Republic of China - 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Plastic Surgery
Otorhinolaryngology - 出版者：Springer New York
- ISSN：1432-5241
文摘
Background Syngeneic adipose-derived stem cells (ASCs) promote the survival of fat grafts. But it is unclear whether allogeneic ASCs have a similar protective effect. In this study, we investigated the protective effect of allogeneic ASCs in a fat graft model of immunocompetent rats. Methods Syngeneic and allogeneic ASCs were derived from Lewis (LEW) and Norway-Brown rats, respectively. Fifty-four LEW rats were divided into three groups. Each LEW rat was injected subcutaneously at two paravertebral spots with adipose granules premixed with DMEM (AFT group), syngeneic ASCs (SYNG group), or allogeneic ASCs (ALLG group). Fat grafts were harvested at 7 and 14?days to examine apoptosis rates and immunochemistry staining was performed for Perilipin A and CD34. At 3?months, fat graft volume retentions were measured. The proportion of regulatory T (Treg) cells and the ratio of CD4/CD8 cells in blood were analyzed at 7?days. Results Expression of Perilipin A and CD34 was higher in the ALLG group than the AFT group at 14?days (P?<?0.05). The apoptosis rate in the ALLG group decreased in comparison with the AFT group at 7 and 14?days (P?<?0.05). At 3?months, allogeneic ASCs increased fat graft volume retentions (P?<?0.05). No difference was found in the proportion of Treg cells and CD4/CD8 cells ratio between groups. There were no statistically significant difference between ALLG and SYNG groups at all time points (P?>?0.05). Conclusions Allogeneic ASCs protected fat grafts at the early stage and improved long-term volume retention in the fat graft model of immunocompetent rats with no or little obvious immune rejection. No Level Assigned This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.?springer.?com/-0266.