Resiquimod and polyinosinic–polycytidylic acid formulation with aluminum hydroxide as an adjuvant for foot-and-mouth disease vaccine

详细信息    查看全文

• 作者：Chun-Xue Zhou (1)
  Dong Li (1)
  Ying-Li Chen (1)
  Zeng-Jun Lu (1)
  Pu Sun (1)
  Yi-Mei Cao (1)
  Hui-Fang Bao (1)
  Yuan-Fang Fu (1)
  Ping-Hua Li (1)
  Xing-Wen Bai (1)
  Bao-Xia Xie (1)
  Zai-Xin Liu (1)
  
• 关键词：R848 ; Poly(I ; C) ; Aluminum hydroxide ; FMDV
• 刊名：BMC Veterinary Research
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：10
• 期：1
• 全文大小：318 KB
• 作者单位：Chun-Xue Zhou (1)
  Dong Li (1)
  Ying-Li Chen (1)
  Zeng-Jun Lu (1)
  Pu Sun (1)
  Yi-Mei Cao (1)
  Hui-Fang Bao (1)
  Yuan-Fang Fu (1)
  Ping-Hua Li (1)
  Xing-Wen Bai (1)
  Bao-Xia Xie (1)
  Zai-Xin Liu (1)
  
  1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of Agriculture, OIE/National Foot-and-Mouth Disease Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China
  
• ISSN：1746-6148

文摘

Background Toll-like receptor (TLR) agonists reportedly have potent antiviral and antitumor activities and may be a new kind of adjuvant for enhancing immune efficacy. Resiquimod (R848) is an imidazoquinoline compound with potent antiviral activity and functions through the TLR7/TLR8 MyD88-dependent signaling pathway. Polyinosinic-polycytidylic acid [poly(I:C)] is a synthetic analog of double-stranded RNA that induces the production of pro-inflammatory cytokines by the activation of NF-κB?through TLR3. This study investigated the potential of R848 and poly(I:C) as an adjuvant 146S foot-and-mouth disease virus (FMDV) vaccine formulated with aluminum hydroxide (Al(OH)3). Results Antibody titers to FMDV and CD8+ T cells were markedly enhanced in mice immunized to 146S?FMDV-?Al(OH)3-?R848-?poly(I:C) compared with mice immunized to FMDV-?ISA206. IFN-γ secretion substantially increased compared with IL-4 secretion by splenic T cells stimulated with FMDV antigens in vitro, suggesting that R848, poly(I:C), and with Al(OH)3 together biased the immune response toward a Th1-type direction. Conclusions These results indicated that the R848 and poly(I:C) together with Al(OH)3 enhanced humoral and cellular immune responses to immunization with 146S?FMDV antigens. Thus, this new vaccine formulation can be used for FMDV prevention.