Identification of phase and sex-related ISSR markers of red algaGracilaria lemaneiformis

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• 作者：Sun Xue (1) (2)
  Zhang Xuecheng (1)
  Mao Yunxiang (1)
  Sui Zhenghong (1)
  Qin Song (3)
  
• 关键词：Gracilaria lemaneiformis ; phase ; sex ; polymorphism ; ISSR
• 刊名：Journal of Ocean University of China
• 出版年：2006
• 出版时间：January 2006
• 年：2006
• 卷：5
• 期：1
• 页码：82-84
• 全文大小：336KB
• 参考文献：1. Blair, M. W., O. Panaud, and S. R. McCouch, 1999. Inter-simple sequence repeats (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice ( / Oryza sativa L.). / Theor. Appl. Genet.,98: 780鈥?92. CrossRef
  2. Kantety, R. V., X. Zeng, and J. Bennetzen, 1995. Assessment of genetic diversity in popcorn ( / Zea mays L.) inbred lines using inter-simple sequence repeat (ISSR) amp-lification. / Mol. Breed,1: 365鈥?73. CrossRef
  3. Kojima, T., T. Nagaoka, and K. Noda, 1998. Genetic linkage map of ISSR and RAPD markers in Einkorn wheat in relation to that of RFLP markers. / Theor. Appl. Genet.,96: 37鈥?5. CrossRef
  4. Lee, Y. K., S. H. Kim, C. B. Hong, O. K. Chah, G. H. Kim, et al., 1998. Heat-shock protein 90 may be involved in differentiation of the female gametophytes in / Griffithsia japonica (Ceramiales, Rhodophyta). / J. Phycol.,34: 1017鈥?023. CrossRef
  5. Li, X. F., Z. H. Sui, and X. C. Zhang, 1998. Application of RAPD in genetic diversity study on / Gracilaria lemaneiformis III. Phase and sex related markers. / Chin. J. Oceanol. Limnol.,16 (Suppl.): 147鈥?51. CrossRef
  6. Liu, Q. Y., S. L. Baldauf, and M. E. Reith, 1996. Elongation factor 1 alpha genes of red alga / Porphyra purpurea include a novel, developmentally specialized variant. / Plant Mol. Biol.,31: 77鈥?5. CrossRef
  7. Liu, Q. Y., and M. E. Reith, 1994a. Isolation of a gametophyte-specific cDNA encoding a lipoxygenase from the red alga / Porphyra purpurea. / Mol. Mar. Biol. Biotechnol.,3: 206鈥?09.
  8. Liu, Q. Y., J. P. van der Meer, and M. E. Reith, 1994b. Isolation and characterization of phase-specific complementary DNAs from sporophytes and gametophytes of / Porphyra purpurea (Rhodophyta) using subtracted complementary DNA libraries. / J. Phycol.,30: 513鈥?20. CrossRef
  9. Martinez, E. A., M. C. Destombe, M. C. Quillet, and M. Valero, 1999. Identification of random amplified polymorphic DNA (RAPD) markers highly linked to sex determination in the red alga / Gracilaria gracilis. / Mol. Ecol.,8: 1533鈥?538. CrossRef
  10. Moreno, S., J. P. Martin, and M. Ortiz, 1998. Inter-simple sequence repeats PCR for characterization of closely related grapevine germplasm. / Euphytica,101: 117鈥?25. CrossRef
  11. Sha, Z. X., X. C. Zhang, and Z. E. Li, 1996, Comparative studies on agar yields and properties of / Gracilaria lemaneiformis at different life stages and in different seasons. / Fresh. Fish. 26 (Suppl.): 334鈥?38 (in Chinese).
  12. Sun, X., X. C. Zhang, Y. X. Mao, J. J. Liu, Z. H. Sui, / et al., 2003. ISSR analysis of marine red algae / Gracilaria (Rhodophyceae). / High Technol. Lett.,13: 89鈥?3 (in Chinese).
  13. Sun, X., G. P. Yang, Y. X. Mao, X. C. Zhang, Z. H. Sui, / et al., 2002. Analysis of expressed sequence tags of a marine alga. / Gracilaria lemaneiformis. Prog. Nat. Sci.,12: 518鈥?23.
  14. Tautz, D., and M. Renz, 1984. Simple sequences are ubiquitous repetitive components of eukaryotic genomes. / Nucleic Acids Res.,12: 4127鈥?138. CrossRef
  15. Vis, M. L., 1999. Inter-simple sequence repeats (ISSR) molecular markers to distinguish gametophytes of / Batrachospermum boryanum (Batrachospermales, Rhodophyta). / Phycologia,38: 70鈥?3. CrossRef
  16. Zhang, X. C., Z. H. Sui, X. F. Li, X. J. Cheng, Z. X. Sha, / et al., 1999. New progress in research of / Gracilaria lemaneiformis. In: / Germplasm Biology on Commercial Seaweeds, Tseng, C. K., ed., Shandong Technology Publication, Jinan, 91鈥?8.
  17. Zietkiewicz, E., A. Rafalksi, and D. Labuda, 1994. Genome fingerprinting by simple sequence repeats (SSR) anchored polymerase chain reaction amplification. / Genomics,20: 176鈥?83. CrossRef
  
• 作者单位：Sun Xue (1) (2)
  Zhang Xuecheng (1)
  Mao Yunxiang (1)
  Sui Zhenghong (1)
  Qin Song (3)
  
  1. College of Marine Life Sciences, Ocean University of China, 266003, Qingdao, P. R. China
  2. Key Laboratory of Marine Biotechnology, Ningbo University, 315211, Ningbo, P. R. China
  3. Institute of Oceanology, Chinese Academy of Sciences, 266071, Qingdao, P. R. China
  
• ISSN：1993-5021

文摘

Six ISSR primers are employed to display the polymorphism of different phases and sexes of red algaGracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by primer P1 of the female gametophyte is identical to that of tetrasporophyte, but distinct from that of male gametophyte. Of the bands produced by primer P3, one is specific to female gametophyte. Three morphologically similar fronds can be easily identified using ISSR technique. Two specific markers, SM1 and SF3, related to male gametophyte and female gametophyte, are cloned and sequenced. The homologous sequences of SM1 are found to encode a hypothetical protein. There is no homologous sequence of SF3 that can be found in GenBank.