A study of the interaction between enantiomers of zolmitriptan and hydroxypropyl-beta-cyclodextrin by capillary electrophoresis

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• 作者：Nannan Pang ; Zhengxiang Zhang ; Yu Bai and Huwei Liu
• 关键词：Binding constant ; Capillary zone electrophoresis ; Enantioseparation ; Zolmitriptan ; Cyclodextrin
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2009
• 出版时间：January, 2009
• 年：2009
• 卷：393
• 期：1
• 页码：313-320
• 全文大小：212.3 KB

文摘

The enantioresolution of zolmitriptan was performed using cyclodextrin (CD)-modified capillary zone electrophoresis (CZE) with hydroxypropyl-β-CD (HP-β-CD) as the chiral selector. The influence of experimental conditions on the enantioseparation of zolmitriptan, such as pH, temperature, applied voltage, and concentrations of running electrolyte and CD, was systematically investigated, obtaining a baseline separation of two enantiomers by the use of a 25 mM sodium dihydrogen phosphate (SDPH) running electrolyte (pH 2.4) containing 30 mM HP-β-CD at 15 ¡ãC. Binding constants for each enantiomer–HP-β-CD pair at different temperatures, as well as thermodynamic parameters for binding, were calculated. A nonlinear van’t Hoff plot was obtained, indicating that the thermodynamic parameters of complexation were temperature-dependent for zolmitriptan enantiomers. The significant contribution of the enthalpy difference to the Gibbs free energy change suggested a stereomeric barrier mechanism for chiral recognition.