Comparative characterization of microRNAs in Schistosoma japonicum schistosomula from Wistar rats and BALB/c mice

详细信息    查看全文

• 作者：Hongxiao Han ; Jinbiao Peng ; Yang Hong ; Zhiqiang Fu ; Ke Lu ; Hao Li…
• 关键词：MicroRNA (miRNA) ; Schistosoma japonicum (S. japonicum) ; Wistar rats
• 刊名：Parasitology Research
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：114
• 期：7
• 页码：2639-2647
• 全文大小：456 KB
• 参考文献：Ambros V (2003) MicroRNA pathways in flies and worms: growth, death, fat, stress, and timing. Cell 113(6):673-76PubMed View Article
  Cai P et al (2011) Profiles of small non-coding RNAs in Schistosoma japonicum during development. PLoS Negl Trop Dis 5(8):e1256PubMed Central PubMed View Article
  Cai P et al (2013) A deep analysis of the small non-coding RNA population in Schistosoma japonicum eggs. PLoS One 8(5):e64003PubMed Central PubMed View Article
  Carthew RW, Sontheimer EJ (2009) Origins and mechanisms of miRNAs and siRNAs. Cell 136(4):642-55PubMed Central PubMed View Article
  Cheng G, Jin Y (2012) MicroRNAs: potentially important regulators for schistosome development and therapeutic targets against schistosomiasis. Parasitology 139(5):669-79PubMed View Article
  Cheng G, Luo R, Hu C, Cao J, Jin Y (2013) Deep sequencing-based identification of pathogen-specific microRNAs in the plasma of rabbits infected with Schistosoma japonicum. Parasitology 140(14):1751-761PubMed View Article
  de Souza Gomes M, Muniyappa MK, Carvalho SG, Guerra-Sa R, Spillane C (2011) Genome-wide identification of novel microRNAs and their target genes in the human parasite Schistosoma mansoni. Genomics 98(2):96-11PubMed View Article
  Devaney E, Winter AD, Britton C (2010) microRNAs: a role in drug resistance in parasitic nematodes? Trends Parasitol 26(9):428-33PubMed Central PubMed View Article
  Guo H, Ingolia NT, Weissman JS, Bartel DP (2010) Mammalian microRNAs predominantly act to decrease target mRNA levels. Nature 466(7308):835-40PubMed Central PubMed View Article
  Han H et al (2012) Molecular cloning and characterization of a cyclophilin A homologue from Schistosoma japonicum. Parasitol Res 111(2):807-17PubMed View Article
  Han H et al (2013a) Apoptosis phenomenon in the schistosomulum and adult worm life cycle stages of Schistosoma japonicum. Parasitol Int 62(2):100-08PubMed View Article
  Han H et al (2013b) Comparison of the differential expression miRNAs in Wistar rats before and 10?days after S. japonicum infection. Parasit Vectors 6:120PubMed Central PubMed View Article
  He YX, Salafsky B, Ramaswamy K (2001) Host–parasite relationships of Schistosoma japonicum in mammalian hosts. Trends Parasitol 17(7):320-24PubMed View Article
  Hoefig KP, Heissmeyer V (2008) MicroRNAs grow up in the immune system. Curr Opin Immunol 20(3):281-87PubMed View Article
  Huang da W, Sherman BT, Lempicki RA (2009) Bioinformatics enrichment tools: paths toward the comprehensive functional analysis of large gene lists. Nucleic Acids Res 37(1):1-3PubMed Central PubMed View Article
  Kim VN (2005) MicroRNA biogenesis: coordinated cropping and dicing. Nat Rev Mol Cell Biol 6(5):376-85PubMed View Article
  Liu F et al (2006) New perspectives on host-parasite interplay by comparative transcriptomic and proteomic analyses of Schistosoma japonicum. PLoS Pathog 2(4):e29PubMed Central PubMed View Article
  Peng J et al (2011a) Apoptosis governs the elimination of Schistosoma japonicum from the non-permissive host Microtus fortis. PLoS One 6(6):e21109PubMed Central PubMed View Article
  Peng J et al (2011b) Differential gene expression in Schistosoma japonicum schistosomula from Wistar rats and BALB/c mice. Parasit Vectors 4:155PubMed Central PubMed View Article
  Sayed D, Abdellatif M (2011) MicroRNAs in development and disease. Physiol Rev 91(3):827-87PubMed View Article
  Silva-Leitao FW, Biolchini CL, Neves RH, Machado-Silva JR (2009) Development of Schistosoma mansoni in the laboratory rat analyzed by light and confocal laser scanning microscopy. Exp Parasitol 123(4):292-95PubMed View Article
  Sun J, Wang S, Li C, Ren Y, Wang J (2014) Novel expression profiles of microRNAs suggest that specific miRNAs regulate gene expression for the sexual maturation of female Schistosoma japonicum after pairing. Parasit Vectors 7:177PubMed Central PubMed View Article
  Wang Z et al (2010) An "in-depth" description of the small non-coding RNA population of Schistosoma japonicum schistosomulum. PLoS Negl Trop Dis 4(2):e596PubMed Central PubMed View Article
  Xiao B et al (2009) Induction of microRNA-155 during Helicobacter pylori infection and its negative regulatory role in the inflammatory response. J Infect Dis 200(6):916-25PubMed View Article
  Xu L et al (2013) Genome-wide identification and characterization of cadmium-responsive microRNAs and their target genes in radish (Raphanus sativus L.) roots. J Exp Bot 64(14):4271-287PubMed Central PubMed View Article
  Xu X et al (2014) Serodiagnosis of Schistosoma japonicum infection: genome-wide identification of a protein marker, and assessment of its diagnostic validity in a field study in China. Lancet Infect Dis 14(6):489-97PubMed View Article
  You H, McManus DP, Hu W, Smout MJ, Brindley PJ, Gobert GN (2013) Transcriptional responses of in vivo praziquantel exposure in schistosomes identifies a functional role for calcium signall
• 作者单位：Hongxiao Han (1) (4)
  Jinbiao Peng (2)
  Yang Hong (1)
  Zhiqiang Fu (1)
  Ke Lu (1)
  Hao Li (1)
  Chuangang Zhu (1)
  Qiuhua Zhao (4)
  Jiaojiao Lin (1) (3)
  
  1. Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology, Ministry of Agriculture, 518 Ziyue Road, Minhang, Shanghai, 200241, China
  4. Minhang Animal Disease Control Center, Shanghai, 201109, China
  2. Shanghai Public Health Clinical Center, Fudan University, Shanghai, 201508, China
  3. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Microbiology
  Immunology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-1955

文摘

More than 40 kinds of mammals in China are known to be naturally infected with Schistosoma japonicum (S. japonicum) (Peng et al. Parasitol Res 106:967-6, 2010). Compared with permissive BALB/c mice, rats are less susceptible to S. japonicum infection and are considered to provide an unsuitable microenvironment for parasite growth and development. MicroRNAs (miRNAs), via the regulation of gene expression at the transcriptional and post-transcriptional levels, may be responsible for developmental differences between schistosomula in these two rodent hosts. Solexa deep-sequencing technology was used to identify differentially expressed miRNAs from schistosomula isolated from Wistar rats and BALB/c mice 10?days post-infection. The deep-sequencing analysis revealed that nearly 40?% of raw reads (10.37 and 10.84 million reads in schistosomula isolated from Wistar rats and BALB/c mice, respectively) can be mapped to selected mirs in miRBase or in species-specific genomes. Further analysis revealed that several miRNAs were differentially expressed in schistosomula isolated from these two rodents; 18 were downregulated (by <2-fold) and 23 were up-regulated (>2-fold) (expression levels in rats compare with those in mice). Additionally, three novel miRNAs were primarily predicted and identified. Among the 41 differentially expressed miRNAs, 4 miRNAs had been identified with specific functions in schistosome development or host-parasite interaction, such as sexual maturation (sja-miR-1, sja-miR-7-5p), embryo development (sja-miR-36-3p) in schistosome, and pathogenesis of schistosomiasis (sja-bantam). Then, the target genes were mapped, filtered, and correlated with a set of genes that were differentially expressed genes in schistosomula isolated from mice and rats, which we identified in a S. japonicum oligonucleotide microarray analysis in a previous study. Gene Ontology (GO) analysis of the predicted target genes of 13 differentially expressed miRNAs revealed that they were involved in some important biological pathways, such as metabolic processes, the regulation of protein catabolic processes, catalytic activity, oxidoreductase activity, and hydrolase activity. The study presented here includes the first identification of differentially expressed miRNAs between schistosomula in mice or rats. Therefore, we hypothesized that the differentially expressed miRNAs may affect the development, growth, and maturation of the schistosome in its life cycle. Our analysis suggested that some differentially expressed miRNAs may impact the survival and development of the parasite within a host. This study increases our understanding of schistosome development and host-parasite interactions.