Alkaloid variations in Catharanthus roseus seedlings treated by different temperatures in short term and long term

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• 作者：Xiao-rui Guo (1)
  Lei Yang (1)
  Jing-hua Yu (1)
  Zhong-hua Tang (1)
  Yuan-gang Zu (1)
  
• 关键词：Catharanthus roseus ; Temperature ; Treatment time ; Alkaloids
• 刊名：Journal of Forestry Research
• 出版年：2007
• 出版时间：December 2007
• 年：2007
• 卷：18
• 期：4
• 页码：313-315
• 全文大小：230KB
• 参考文献：1. Aert, R.J., Baumann, T.W., Slusarenko, A. Sch?fer, A., Hesse, M. 1996. Signaling molecules and the synthesis of alkaloids in / Catharanthus roseus seedlings. / Phytochemistry, 42: 417-22. CrossRef
  2. Datta, A., Sricastava, P.S. 1997. Variation in vinblastine production by / Catharanthus roseus during / in vivo and / in vitro differentiation. / Phytochemistry, 46: 135-37 CrossRef
  3. De Luca, V. and St-Pierre, B. 2000. The cell and developmental biology of alkaloid biosynthesis. / Trends in Plant Science, 4: 168-73. CrossRef
  4. Facchini, P.J. 2001. Alkaloid biosynthesis in plants: biochemistry, cell biology, molecualr regulation and metabolic engineering applications. / Annual Review Plant Physiology Plant Molecular Biology, 52: 29-6. CrossRef
  5. Frischknecht, P.M., Battig, M., Baumann, T. 1987. Effect of drought and wounding stress on indole alkaloid formation in / Catharanthus roseus. / Phytochemistry, 26: 707-10 CrossRef
  6. Gao Yang, Zhang Xue-ke, Guo Xiao-rui, Sun Yan-fei, Zu Yuan-gang. 2006. Effects of tip-pruning treatment on source-sink regulation of / Catharanthus roseus seedlings. / Journal of Forestry Research, 17: 326-28. CrossRef
  7. Hashimoto, T. and Yamada, Y. 1994. Alkaloid biogenesis: Molecular aspects. / Annual Review Plant Physiology Plant Molecular Biology, 45: 257-85.
  8. Sottomayor, M., De Pinto, M.C., Salema, R., Dicosmo, F., Pedre?o, A, Ros Barcelo, A. 1996. The vacuolar localization of a basic peroxidase isoenzyme responsible for the synthesis of α-3- 4-anhydrovinblastine in / Catharanthus roseuss (L.) G. Don leaves. / Plant Cell Environment, 19: 761-67. CrossRef
  9. St-Pierre, B., Vazquez-Flota, F.A., De Luca, V. 1999. Multicellular compartmentation of / Catharanthus roseus alkaloid biosynthesis predicts intercellular translocation of a pathway intermediate. / The Plant Cell, 11: 887-00. CrossRef
  10. Vazquez-Flota, F., Carrillo-Pech, M., Minero-García, Y., De Lourdes Miranda-Ham, M. 2004. Alkaloid metabolism in wounded / Catharanthus roseus seedlings. / Plant Physiology and Biochemistry, 42: 623-28. CrossRef
  11. Zu Yuan-gang, Tang Zhong-hua, Yu Jing-hua, Liu Shi-gang, Wang Wei, Guo Xiao-rui. 2003. Different responses of camptothecin and 10-hydroxycamptothecin to heat shock in / Camptotheca acuminata seedlings. / Journal of Integrative Plant Biology, 7: 809-14.
  
• 作者单位：Xiao-rui Guo (1)
  Lei Yang (1)
  Jing-hua Yu (1)
  Zhong-hua Tang (1)
  Yuan-gang Zu (1)
  
  1. Key Laboratory of Forest Plant Ecology, the Ministry of Education of China, Northeast Forestry University, Harbin, 150040, P. R. China
  

文摘

A study was conducted to investigate the effects of high temperature on variations of alkaloid metabolism in C. roseus seedlings in Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Heilongiang, China. 60-day-old C. roseus seedlings with 3- pairs of leaves were incubated in chambers with temperature of 30°C and 40°C for short-term heat shock experiment and 20°C, 25°C and 35°C for long-term experiment. The contents of vindoline, catharanthine, vinblastine and vincristine in C. roseus leaves and root were checked at different temperatures in short term (1- h) and long term (1-6 d). Results showed that under short-term heat shock, the contents of vindoline, catharanthine and vinblastine in leaves of the seedlings were higher at 40°C than at 30°C, but after 6 h treatment, the contents of vindoline and catharanthine under the two temperatures came to the same level. Catharanthine was exclusively distributed in C. roseus roots and its content was increased by 40% after two hours incubation at 40°C, while increased slowly at 30°C incubation and reached the highest value at 6 h. In the Long-term experiment, concentrations of monomeric alkaloids catharanthine and vindoline were higher at 20°C than at 25°C and had a sharp increase under the condition of 35°C. While for dimeric alkaloid, it showed that the higher the temperature, the earlier the peak value of vinblastine content appears. Vincristine had a continuous enhancement and attained 0.027 mg·g? at 16th day under 35°C condition which was higher than those in the other conditions. It was concluded that high temperature could promote the accumulation of different alkaloids in C. roseus and the accumulation characteristic is highly related to treatment time.