The interactions between bovine serum albumin and carboxybetaine-functionalized polysiloxanes in solution
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- 作者：Yaling Lin ; Weiyan Huang ; Yang You ; Yufeng Lei…
- 关键词：Carboxybetaine functionalized polydimethylsiloxanes ; Protein ; Polymer/protein interactions ; Fluorescence spectroscopy ; Isothermal titration calorimetry
- 刊名：Colloid and Polymer Science
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：294
- 期：12
- 页码：2029-2037
- 全文大小：
- 刊物类别：Chemistry and Materials Science
- 刊物主题：Polymer Sciences; Soft and Granular Matter, Complex Fluids and Microfluidics; Characterization and Evaluation of Materials; Physical Chemistry; Food Science; Nanotechnology and Microengineering;
- 出版者：Springer Berlin Heidelberg
- ISSN：1435-1536
- 卷排序：294
文摘
The interactions between carboxybetaine-functionalized polydimethylsiloxanes (PDMS-g-CB) and bovine serum albumin (BSA) in water solution were investigated from varied aspects based on different techniques, including fluorescence spectroscopy, UV-Vis spectroscopy, circular dichroism spectroscopy, isothermal titration calorimetry, and atomic force microscopy. Due to the weak interaction between PDMS-g-CB and BSA, another polymer with similar structure as PDMS-g-CB, i.e., quaternary ammonium salt-functionalized polydimethylsiloxanes (PDMS-g-QAS), was synthesized and chosen as the positive control, and the results indicated that the free PDMS-g-CB in solution could exhibit good resistance against proteins, which was mainly due to the zwitterions-side groups’ binding ability between PDMS chain and protein. Both the characterization methods and the results could help us to understand the interaction between free water-soluble polymers and protein, and extend the application of PDMS-g-CB and design new antifouling materials.