CD133+CD34+ and CD133+CD38+ blood progenitor cells as predictors of platelet engraftment in patients undergoing autologous peripheral blood stem cell transplantation

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• 作者：Alma Y. Camacho Villa^a ; Elba Reyes Maldonado^a ; Laura A. Montiel Cervantes^a ; ^b ; Jorge Vela Ojeda^a ; ^b ; ^{velaj12x@prodigy.net.mx} ; ^{jorge.vela@imss.gob.mx}
• 关键词：Autologous transplantation ; Stem cells ; Engraftment ; CD133+CD38+ cells ; CD133+CD34+ cells
• 刊名：Transfusion and Apheresis Science
• 出版年：2012
• 期刊代码：248_14730502
• 类别：med
• 出版时间：June, 2012
• 卷：46
• 期：3
• 页码：239-244
• 文件大小：323 K

摘要

Background

Hematopoietic stem cells (HSC) have been characterized by CD34+ expression and an adequate dose of CD34+ cells is associated with a complete engraftment. CD133 is a more specific marker of HSC.

Materials and methods

We studied the relationship between graft content of CD34+, CD133+, and CD38+ cells and trilineage engraftment after autologous stem cell transplantation in patients with different hematological disorders. Blood samples were obtained before and after mobilization with recombinant granulocyte-colony stimulating factor (G-CSF, 16 渭g/kg), from apheresis collections, and after transplantation.

Results

Cell subsets were quantified by flow cytometry, and the dose of each population infused was correlated with success of engraftment. G-CSF induced mobilization of CD133+CD38+ cells (12.6-fold) and CD133+CD34+ cells (14.7-fold). A correlation was observed between the infused dose of CD133+CD34+ and CD133+CD38+ cells and platelet engraftment.

Conclusion

CD133+CD34+ and CD133+CD38+ cells were mobilized with G-CSF and these cell subsets were correlated with platelet engraftment.