Development and characterization of a monoclonal antibody against porcine CD205

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• 作者：Lilian Flores-Mendoza^a ; ^b ; Carlos Velazquez^c ; Jocelyn Bray^d ; Leo Njongmeta^d ; Waithaka Mwangi^d ; Jesú ; s Herná ; ndez^a ; ^{jhdez@ciad.mx}
• 关键词：Dendritic cells ; C-type lectin receptors ; CD205 ; Porcine
• 刊名：Veterinary Immunology and Immunopathology
• 出版年：2012
• 期刊代码：105_01652427
• 类别：abs
• 出版时间：15 March, 2012
• 卷：146
• 期：1
• 页码：74-80
• 文件大小：767 K

摘要

The aim of this work was to develop mAbs against porcine CD205 and to conduct a comparative analysis of the CD205 protein expression on lymphoid tissues, monocyte-derived dendritic cells (DCs) and DCs isolated from the porcine skin. To conduct this study, we generated a monoclonal antibody, designated 1.F6F6, against the C-type lectin-like domain-5 of the porcine CD205 and showed that it recognizes a protein band of 鈭?00 kDa by Western Blot analysis in mesenteric lymph nodes cells. Flow cytometric analysis showed that the mAb 1.F6F6 recognized 28.5%, 28.1%and 34.1%of cells from tonsil, inguinal and mesenteric lymph nodes, respectively, and 6%of cells from thymus. Analysis of monocyte-derived DCs showed that approximately 20%were positive and activation of the cells with LPS increased the positive population to 36%. Analysis of DCs isolated from the porcine skin showed that approximately 70%of the cell population expressed the CD205 receptor. The development of a monoclonal antibody capable of recognizing the CD205 receptor in swine opens up possibilities of applying new strategies for enhancing vaccine efficacy by using the anti-CD205 antibody for DC antigen-targeting to enhance priming of immune responses.