Association of SLC11A1 (NRAMP1) polymorphisms with pulmonary Mycobacterium avium complex infection

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• 作者：Bishwa Raj Sapkota^a ; ^b ; Minako Hijikata^a ; Ikumi Matsushita^a ; Goh Tanaka^c ; Ryuji Ieki^d ; ^e ; Nobuyuki Kobayashi^f ; Emiko Toyota^g ; Hideaki Nagai^g ; Atsuyuki Kurashima^g ; ^h ; ⁱ ; Katsushi Tokunaga^b ; Naoto Keicho^a ; ^{keicho@ri.ncgm.go.jp}
• 关键词：Mycobacterium avium complex ; SLC11A1 ; NRAMP1 ; Genetic polymorphisms ; Allelic expression imbalance
• 刊名：Human Immunology
• 出版年：2012
• 期刊代码：75_01988859
• 类别：bio
• 出版时间：May, 2012
• 卷：73
• 期：5
• 页码：529-536
• 文件大小：923 K

摘要

Although genetic variants in SLC11A1 (NRAMP1) have been associated with mycobacterial diseases, these findings have not been extensively validated in pulmonary Mycobacterium avium complex (MAC) infection. This study investigated the genomic structure of SLC11A1 and its association with MAC infection. Nineteen polymorphic loci were genotyped in European descendents and the Japanese population. Linkage disequilibrium (LD) structures and frequencies of major haplotypes differed between these 2 populations. Tag single nucleotide polymorphisms (SNPs) were chosen from the data set, and 6 polymorphic sites were genotyped in 122 pulmonary MAC cases and 211 controls from Japan. We observed that the T allele of rs2279014 in the 3鈥?untranslated region was associated with protection from MAC disease when comparing allele frequencies with an odds ratio of 0.582 (95%confidence interval 0.379-0.894, p = 0.013). The frequencies of haplotypes constructed with the above 6 variants did not differ between cases and controls. Allele-specific expression imbalance of SLC11A1 mRNA was evaluated in peripheral blood cells from heterozygous individuals, but no difference was observed among haplotypes. Although the significance was modest, rs2279014 is in strong LD with nearby SNPs and further studies are required for conclusive validation.