In vitro propagation and molecular evaluation of a Capsicum annuum L. cultivar with a high chromosome number (2n = 48)

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• 作者：Arpita Dafadar ; Anusree Das ; Subhendu Bandyopadhyay ; Timir baran Jha ; ^{tbjha2000@yahoo.co.in} ; ^{presibot@vsnl.net}
• 关键词：Capsicum annuum ; Chromosome number 2n  ; =  ; 2x  ; =  ; 48 ; Indirect organogenesis ; RAPD ; Clonal fidelity
• 刊名：Scientia Horticulturae
• 出版年：2012
• 期刊代码：93_03044238
• 类别：abs
• 出版时间：1 June, 2012
• 卷：140
• 期：Complete
• 页码：119-124
• 文件大小：956 K

摘要

Aseptic leaf explants of a Capsicum annuum with 2n = 2x = 48 chromosomes were used for the first time to produce genetically uniform plants. In vitro shoot organogenesis from leaf explants was achieved on Murashige and Skoog's (MS) medium with different concentration and combinations of BA (6.66-44.38 渭M), Kn (2.32 渭M), GA₃ (5.20-13.00 渭M) and IAA (2.85-5.71 渭M). Maximum number of shoots (6/explants) was obtained in combination of 8.87 渭M BAP and 2.85 渭M IAA. Complete plants were produced on MS basal medium. Hardened plants survived under field conditions and flowered with viable first generation seeds (R₁) within one year at the tropical wet-and-dry climate of Kolkata. Somatic chromosome analysis from mother, in vitro regenerants (R₀) and R₁ plants revealed chromosomal homology in all the populations. Gametic chromosome analysis revealed 24 bivalents and no tetravalent formation in the cultivar, negating the autopolyploid nature of the cultivar. Genomic DNA analyses with RAPD fingerprinting profiles indicate genetic stability and clonal fidelity between the mother, R₀ and R₁ plants.