Enhanced utilization and altered metabolism of arginine in inflammatory macrophages caused by raised nitric oxide synthesis

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• 作者：Mü ; llner ; N&aacute ; ndor ; L&aacute ; z&aacute ; r ; &Aacute ; gnes ; Hrab&aacute ; k ; Andr&aacute ; s
• 关键词：FBS ; foetal bovine serum ; HBSS ; Hank’ ; s balanced salt solution ; LPS ; lipopolysaccharide ; NOS II ; inducible nitric oxide synthase ; PAGE ; polyacrylamide gel electrophoresis ; PBS ; phosphate buffered saline ; PDTC ; pyrrolidine dithiocarbamate ; SDS ; sodium
• 刊名：The International Journal of Biochemistry and Cell Biology
• 出版年：2002
• 期刊代码：127_13572725
• 类别：med
• 出版时间：September, 2002
• 卷：34
• 期：9
• 页码：1080-1090
• 文件大小：188 K

摘要

Nitric oxide (NO) production was increased in macrophages during inflammation. Casein-elicitation of rodents causing a peritoneal inflammation offered a good model to study alterations in the metabolism of l-arginine, the precursor of NO synthesis. The utilization of l-arginine for NO production, arginase pathway and protein synthesis were studied by radioactive labeling and chromatographic separation. The expression of NO synthase and arginase was studied by Western blotting.