Simultaneous determination of water- and fat-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography coupled with diode array detection
- 作者：Klejdus ; Borivoj ; Petrlová ; Jitka ; Pote&scaron ; il ; David ; Adam ; Vojtech ; Mikelová ; Radka ; et. al.
- 关键词：Simultaneous determination ; Vitamins ; Water- and fat-soluble vitamins ; High-performance liquid chromatography ; Pharmaceutical preparations ; Drugs ; Diode array detection ; Mass spectrometry
- 刊名：Analytica Chimica Acta
- 出版年：2004
- 期刊代码：2_00032670
- 类别：ch
- 出版时间：August 23, 2004
- 卷：520
- 期：1-2
- 页码：57-67
- 文件大小：348 K
摘要
Water- and fat-soluble vitamins were separated on a MetaChem Polaris C18-A (150mm×4.6mm, 3μm particle size) in a single run using combined isocratic and linear gradient elution with a mobile phase consisting of 0.010%trifluoroacetic acid of pH 3.9 (solvent A) and methanol (solvent B) at the flow rate 0.7mlmin−1. A linear gradient profile (A:B) started at 95:5 and was constant in the first 4min, then linearly decreased up to 2:98 during the next 6min, then it was constant in the next 20min and finally linearly increased up to 95:5 ratio of water phase in the last 5min of the separation. The most suitable detection wavelength for simultaneous vitamin determination was 280nm. The method was applied for the solid sample of pharmaceutical preparation (B-Komplex), fortified powdered drinks (multi-vitamin) and food samples. The results were in good agreement with the declared values.