Polysaccharides from the Styrian oil-pumpkin with antioxidant and complement-fixing activity

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• 作者：Zuzana Ko&scaron ; 钮&aacute ; lov&aacute ; ^a ; ^{chemzuko@savba.sk} ; Zdenka Hrom&aacute ; dkov&aacute ; ^a ; Anna Ebringerov&aacute ; ^a ; Martin Polovka^b ; Terje Einar Michaelsen^c ; Berit Smestad Paulsen^c
• 关键词：Oil-pumpkin ; Pectin ; Structure ; Antioxidant ; Immunomodulatory activity
• 刊名：Industrial Crops and Products
• 出版年：2013
• 期刊代码：56_09266690
• 类别：abs
• 出版时间：January, 2013
• 卷：41
• 期：Complete
• 页码：127-133
• 文件大小：880 K

摘要

Pectic polysaccharides have attracted great attention due to their health-promoting potential. From the Styrian oil-pumpkin biomass representing an alternative pectin source, two series of acidic polysaccharide fractions were isolated using in succession hot water, EDTA and dilute HCl. Chemical and spectroscopic (FTIR, NMR) analyses of the fractions revealed the predominance of partially methylesterified and acetylated pectins containing homogalacturonan and ramified rhamnogalacturonan elements, and a minority of phenolic compounds, protein and hemicelluloses. The pectins exhibited moderate antioxidant activities tested by colorimetric DPPH and FRAP assays and EPR method. The activities correlated with the total phenolic content. In the complement-fixing test, most of the pectin fractions exhibited potent effects comparable to the positive control - a pectin from Plantago major. The oil-pumpkin pectins represent potential antioxidant and immunoenhancing additives applicable in food and nutraceuticals.