Enhanced in vitro propagation of Miscanthus 脳 giganteus

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• 作者：Marcela Gubi&scaron ; ov&aacute ; ^a ; ^b ; Jozef Gubi&scaron ; ^a ; Al啪beta 沤ofajov&aacute ; ^a ; Daniel Mih&aacute ; lik^a ; J&aacute ; n Kraic^a ; ^b ; ^{kraic@vurv.sk}
• 关键词：Miscanthus  ; × ;   ; giganteus ; Micropropagation ; Axillary bud ; Inflorescence ; Callus
• 刊名：Industrial Crops and Products
• 出版年：2013
• 期刊代码：56_09266690
• 类别：abs
• 出版时间：January, 2013
• 卷：41
• 期：Complete
• 页码：279-282
• 文件大小：792 K

摘要

Two approaches for Miscanthus 脳 giganteus in vitro propagation - direct and indirect, were improved as effective alternatives to asexual propagation from rhizomes. The direct multiplication using stem segments containing axillary buds combined with in vitro tillering phase turns out to be fifty-sixty times more effective than the conventional ex vitro rhizome-based approach. On average, 64 regenerants per one immature inflorescence were produced using the indirect system through callus growth and shoots regeneration and inclusion of in vitro tillering increased multiplication by coefficient 2-2.5. Both in vitro systems were improved and could be integrated into effective production of M. 脳 giganteus plantlets.