SFTG international collaborative study on in vitro micronucleus test: III. Using CHO cells
- 作者：Marilyn J. Aardema ; Ronald D. Snyder ; Carol Spicer ; Katyayini Divi ; Takeshi Morita ; Robert J. Mauthe ; David P. Gibson ; Sandra Soelter ; Patrick T. Curry ; Veronique Thybaud et al.
- 关键词：In vitro micronucleus assay ; CHO cells ; Cytochalasin B ; Clastogen ; Aneugen
- 刊名：Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- 出版年：2006
- 期刊代码：31_13835718
- 类别：pha
- 出版时间：4 August 2006
- 卷：607
- 期：1
- 页码：61-87
- 文件大小：233 K
摘要
In this report, results are presented from an international study of the in vitro micronucleus assay using Chinese hamster ovary cells. This study was coordinated by an organizing committee supported by the SFTG (the French branch of the European Environmental Mutagen Society). Test chemicals included mannitol, bleomycin, cytosine arabinoside, urethane and diethylstilboestrol. Mitomycin C was used as a positive control. Each chemical was evaluated in at least two laboratories following a variety of different protocols (short and long exposures, varying recovery times, with and without cytochalasin B) in order to help determine a standard protocol for routine testing in Chinese hamster ovary cells. Mannitol and urethane were negative, while bleomycin, cytosine arabinoside and diethylstilboestrol induced a dose dependent increase in micronucleated cells. In the presence of cytochalasin B, increases in micronuclei were observed in binucleated as well as mononucleated cells in cultures treated with bleomycin, cytosine arabinoside or diethylstilboestrol. Importantly, all three of these chemicals were detected in each of the different treatment/recovery regimens. No differences were seen in the sensitivity or accuracy of the responses in the presence of absence of cytochalasin B. Overall, these results demonstrate the suitability of Chinese hamster ovary cells for the in vitro micronucleus assay.