IgY14 and SuperMix immunoaffinity separations coupled with liquid chromatography-mass spectrometry for human plasma proteomics biomarker discovery
- 作者：Tujin Shi ; Jian-Ying Zhou ; Marina A. Gritsenko ; Mahmud Hossain ; David G. Camp II ; Richard D. Smith ; Wei-Jun Qian ; weijun.qian@pnnl.gov
- 关键词：HAP ; high-abundance proteins ; MAP ; moderate-abundance proteins ; LAP ; low-abundance proteins ; HSA ; human serum albumin ; IgG ; immunoglobulin G ; SRM ; selected reaction monitoring ; SID ; stable isotope dilution ; MARS ; multiple affinity removal system ; IgY ; imm
- 刊名：Methods
- 出版年：2012
- 期刊代码：103_10462023
- 类别：imm
- 出版时间：February, 2012
- 卷：56
- 期：2
- 页码：246-253
- 文件大小：809 K
摘要
Interest in the application of advanced proteomics technologies to human blood plasma- or serum-based clinical samples for the purpose of discovering disease biomarkers continues to grow; however, the enormous dynamic range of protein concentrations in these types of samples (often >10 orders of magnitude) represents a significant analytical challenge, particularly for detecting low-abundance candidate biomarkers. In response, immunoaffinity separation methods for depleting multiple high- and moderate-abundance proteins have become key tools for enriching low-abundance proteins and enhancing detection of these proteins in plasma proteomics. Herein, we describe IgY14 and tandem IgY14-Supermix separation methods for removing 14 high-abundance and up to 60 moderate-abundance proteins, respectively, from human blood plasma and highlight their utility when combined with liquid chromatography-tandem mass spectrometry for interrogating the human plasma proteome.