Regulatory mechanism of PAC1 gene expression via Sp1 by nerve growth factor in PC12 cells

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• 作者：Ayako Miura ; Naoko Odahara ; Aiko Tominaga ; Kazuhiko Inoue ; Yuki Kambe ; Takashi Kurihara ; Atsuro Miyata ; ^{amiyata@m3.kufm.kagoshima-u.ac.jp}
• 关键词：PACAP ; pituitary adenylate cyclase activating polypeptide ; VIP ; vasoactive intestinal polypeptide ; NGF ; nerve growth factor ; NRSE ; neuron-restrictive silencer elements ; Sp1 ; specificity protein 1 ; Zac1 ; zinc finger protein ; EMSA ; electrophoretic mobility
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：12 June, 2012
• 卷：586
• 期：12
• 页码：1731-1735
• 文件大小：575 K

摘要

In addition to VPAC1 and VPAC2, PAC1 is involved in the pleiotropic action of pituitary adenylate cyclase activating polypeptide (PACAP) in the CNS. A luciferase reporter assay for the human PAC1 gene (鈭?160/+268) revealed that NGF treatment significantly augments the promoter activity of the PAC1 gene. Moreover, the Sp1 site at 鈭?82/鈭?73 was shown to be essential for the NGF-augmented promoter activity of the PAC1 gene. Treatment with U0126, an MEK inhibitor, or Mithramycin A, an Sp1 inhibitor, significantly attenuated promoter activity. These results indicate that activation of Sp1 by the Ras/MAPK pathway might participate in neuron specific expression of the PAC1 gene.