Protective effect of Ornithogalum saundersiae Ait (Liliaceae) against acetaminophen-induced acute liver injury via CYP2E1 and HIF-1伪

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• 作者：Ying WAN^螖 ; Yan-Ling WU^螖 ; Li-Hua LIAN ; Ji-Xing NAN ; ^{jxnanybu@gmail.com}
• 关键词：Ornithogalum saundersiae Ait ; Acetaminophen ; Apoptosis ; Cytochrome P450 2E1 ; Hypoxia-inducible factor-1伪
• 刊名：Chinese Journal of Natural Medicines
• 出版年：2012
• 期刊代码：P72_18755364
• 类别：med
• 出版时间：May, 2012
• 卷：10
• 期：3
• 页码：177-184
• 文件大小：581 K

摘要

Aim

To investigate the hepatoprotective effect of total saponin from Ornithogalum saundersiae Ait (Liliaceae) (OC) against acetaminophen (APAP)-induced acute liver injury in vivo and in vitro.

Methods

Mice were pretreated with OC (300, 200 or 100 mg路kg^鈭?, body weight) or N-acetyl-L-cysteine (NAC) (300 mg路kg^鈭?, body weight) for 3 times at 24 h intervals. APAP was administered 2 h after OC last dose. Chang liver cells were incubated with the medium containing OC (50, 100, 200 mg路mL^鈭?) or NAC (10 mmol路L^鈭?) with the presence or absence of APAP (10 mmol路L^鈭?) for 24 h.

Results

OC showed remarkable hepatoprotective effect 12 h after APAP administration by decreased aspartate aminotransferase and alanine aminotransferase levels, reduced the products of lipid peroxidation, improved the activity of catalase, superoxide dismutase and glutathione peroxidase, inhibited the caspase-3 cleavage and hypoxia inducible factor-1伪 accumulation in vivo. In vitro, OC significantly decreased the activities of metabolism enzyme cytochrome P450 2E1 (CYP2E1) and cyclooxygenase-2 (COX-2) induced by APAP.

Conclusion

OC possesses the ability to protect hepatocyte from APAP-induced liver damage, suggesting that the hepatoprotective mechanism of OC might be related to antioxidation via blocking the CYP2E1, and mediating reactive oxygen species scavenging and accumulation of hypoxia-inducible factor (HIF)-1伪.