Production of acetone-butanol-ethanol from spoilage date palm (Phoenix dactylifera L.) fruits by mixed culture of Clostridium acetobutylicum and Bacillus subtilis

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• 作者：Mohamed Hemida Abd-Alla ; ^{mabdalla@sci.mak.ac.ug} ; ^{mhabdalla2002@yahoo.com} ; Abdel-Wahab Elsadek El-Enany
• 关键词：Acetone&ndash ; butanol&ndash ; ethanol fermentation ; Bacillus subtilis ; Biofuel ; Clostridium acetobutylicum ; Date palm ; Spoilage date fruits
• 刊名：Biomass and Bioenergy
• 出版年：2012
• 期刊代码：3_09619534
• 类别：pw
• 出版时间：July, 2012
• 卷：42
• 期：Complete
• 页码：172-178
• 文件大小：349 K

摘要

Spoilage date palm (Phoenix dactylifera L.) fruits were used as substrate for acetone, butanol and ethanol (ABE) production by mixed culture of Clostridium acetobutylicum ATCC 824 and Bacillus subtilis DSM 4451. B. subitlis was used to consume O₂ and maintain strict anaerobic conditions for ABE production by the strictly anaerobic C. acetobutylicum ATCC 824. The total ABE production of 21.56聽g聽L^鈭? was achieved at 75聽g聽L^鈭? spoilage date fruits homogenate. The maximum ABE productivity of 0.30聽g聽L^鈭?聽h^鈭? and ABE yield of 0.42 were obtained at 75聽g聽L^鈭? spoilage date fruits homogenate by mixed culture without addition of a reducing agent and N₂ flushing. Addition of yeast extract (5聽g聽L^鈭?) or ammonium nitrate (1.6聽g聽L^鈭?) to spoilage date fruits homogenate significantly enhanced ABE production. The combination of yeast extract and ammonium nitrate markedly increased ABE production. These results suggest that spoilage date fruits can be efficiently used for commercial ABE production. The described protocol did not require addition of any costly reducing agent to medium or flushing with N₂ to ensure anaerobic conditions. This will makes the anaerobic fermentation of date fruits homogenate more economical and reduces the cost of ABE production.