Performance of the ABE fermentation and integrated product recovery by cyclic vacuum was investigated in a 14-l bioreactor containing initial glucose concentration of 60聽g聽l鈭? at 35聽掳C. The cyclic operation consisted of 2聽h-vacuum-cycles separated by 4聽h-fermentation intervals at atmospheric pressure. Application of periodic vacuum sufficed to eliminate butanol inhibition of Clostridium beijerinckii 8052 culture, which tolerated alternate vacuum and atmospheric pressures. A 39%reduction in the energy requirement for acetone butanol ethanol (ABE) fermentation and in situ recovery by vacuum was achieved by changing the mode of vacuum operation from continuous (24聽h vacuum time) to cyclic (12聽h vacuum time). Analysis of the overall energy consumption for ABE fermentation and recovery (vacuum fermentation聽+聽distillation) demonstrated that cyclic vacuum ABE fermentation is more energy efficient than the conventional batch fermentation.