The activation, by antigen, of na茂ve TCR transgenic CD4 T cells cultured at physiological, rather than artificially high, frequencies more accurately reflects the in vivo activation of normal numbers of na茂ve CD4⁺ T cells

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• 作者：Christopher D. Rudulier ^{chris.rudulier@usask.ca} ; David R. Kroeger ^{david.kroeger@usask.ca} ; Peter A. Bretscher ; ^{peter.bretscher@usask.ca}
• 关键词：TCR transgenic T cells ; T cell activation ; In vitro
• 刊名：Cellular Immunology
• 出版年：2012
• 期刊代码：279_00088749
• 类别：med
• 出版时间：2012
• 卷：274
• 期：1-2
• 页码：115-120
• 文件大小：738 K

摘要

The majority of in vitro studies investigating the activation of na茂ve TCR transgenic T cells routinely employ an artificially high frequency of such cells. To assess whether employing high frequencies of TCR transgenic cells in vitro accurately reflects the in vivo activation of a normal number of T cells, we cultured between 300 and 3 脳 10⁶ Rag2^{鈭?鈭?/sup> DO11.10 T cells per well under otherwise identical conditions. We find that those T cells cultured at low frequencies proliferate more and are more potently activated, as assessed by the expression of CD44 and CD62L, each giving rise to a much larger number of cytokine producing cells, comparable to the number generated in vivo when a normal number of CD4+ T cells are activated. The effect of T cell frequency on the level of their activation was not due to differences in MHCII or CD80/86 expression by B cells, the major APC population present, nor to increased death of B cells in high frequency cultures. Taken together, our observations illustrate the necessity of culturing na茂ve TCR transgenic CD4+ T cells at a physiological frequency if one is to more accurately recapitulate the in vivo activation of na茂ve CD4+ T cells.}