Differential response of Drosophila cell lines to extracellular adenosine

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• 作者：Jana Fleischmannova^a ; Lucie Kucerova^a ; ^b ; Katerina Sandova^b ; Veronika Steinbauerova^b ; Vaclav Broz^a ; ^b ; Petr Simek^b ; Michal Zurovec^a ; ^b ; ^{zurovec@entu.cas.cz}
• 关键词：Adenosine recycling ; Nucleoside transport ; Mbn2 ; Cl.8+ ; ATP synthesis ; CG11045 ; CG11778 ; CG8083 ; CG11255
• 刊名：Insect Biochemistry and Molecular Biology
• 出版年：2012
• 期刊代码：80_09651748
• 类别：bio
• 出版时间：May, 2012
• 卷：42
• 期：5
• 页码：321-331
• 文件大小：1197 K

摘要

Adenosine (Ado) is a crucial metabolite that affects a wide range of physiological processes. Key proteins regulating Ado signaling, transport and metabolism are conserved among vertebrates and invertebrates. It is well known that Ado influences proliferation of several vertebrate and invertebrate cells. Here we show that Ado negatively influences viability, changes morphology and mitochondrial polarity of the Drosophila imaginal disc cell line (Cl.8+) via a mechanism exclusively dependent on cellular Ado uptake. High transport of Ado is followed by phosphorylation and ATP production as a part of Ado salvation, which at higher concentrations may interfere with cellular homeostasis. In contrast, hematopoietic cell line Mbn2, which grows well in high Ado concentration, preferentially uses adenosine deaminase as a part of the purine catabolic pathway. Our results show that different types of Drosophila cell lines use different pathways for Ado conversion and suggest that such differences may be an important part of complex mechanisms maintaining energy homeostasis in the body.