Differentiation-dependent expression of Adhfe1 in adipogenesis
- 作者：Ji Young Kim ; Kristin S. Tillison ; Shengli Zhou ; Jun Ho Lee ; Cynthia M. Smas
- 关键词：Adipocyte ; Adipose tissue ; 3T3-L1 ; Differentiation ; Adipogenesis ; Iron-activated ; Alcohol dehydrogenase ; Metabolism ; TNFα ; Obesity
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2007
- 期刊代码：116_00039861
- 类别：ch
- 出版时间：1 August 2007
- 卷：464
- 期：1
- 页码：100-111
- 文件大小：1134 K
摘要
We have determined that adipocytes are a major site of expression of the transcript for the novel alcohol dehydrogenase (ADH), Adhfe1. Adhfe1 is unique in that the sequence of its encoded protein places it among the iron-activated ADHs. Western blot analysis reveals Adhfe1 encodes a 50 kDa protein and immunocytochemical staining indicates mitochondrial localization. Adhfe1 transcript exhibits differentiation-dependent expression during in vitro brown and white adipogenesis. Unlike many adipocyte-enriched genes, however, Adhfe1 transcript expression in adipocytes is refractory to TNFα-mediated downregulation. However, use of pharmacological inhibitors reveals PI 3-kinase-mediated signals maintain the basal level of Adhfe1 transcript in 3T3-L1 adipocytes. Tissue profiling studies show Adhfe1 transcript is restricted to white and brown adipose tissues, liver, and kidney. In comparison to C57BL/6 mice, Adhfe1 transcript is downregulated 40%in white adipose tissue of ob/ob obese mice. Further characterization of Adhfe1 should yield new insights into adipocyte function and energy metabolism.