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Copyright © 2008 Published by Elsevier Ltd.
Behavioral improvement induced by bone marrow mesenchymal stem cells in neonatal rats after hypoxic-ischemic brain damage
The radiodensity of the distraction zone was higher in group A than in group B at both time points. Histologically callus was found in both groups but more bone was formed in group A. Histomorphometric analysis also demonstrated that both new bone volume and thickness of the new trabeculae were significantly greater in group A than in group B.
The results of this study suggest that autologous bone marrow stem cell transplantation may be considered as a potential method to accelerate bone regeneration in the distraction gap, and enhance consolidation.
![]() | Identification of senescence Biochemical and Biophysical Research Communications |
![]() Biochemical and Biophysical Research Communications, Volume 371, Issue 3, 4 July 2008, Pages 431-436 Eunsook Ryu, Su Hong, Jaeku Kang, Junghoon Woo, Jungjun Park, Jongho Lee, Jeong-Sun Seo Abstract Human bone marrow mesenchymal stem cells (hBMMSCs) are multipotent stem cells that can differentiate into several specialized cell types, including bone, cartilage, and fat cells. The proliferative capacity of hBMMSCs paves the way for the development of regenerative medicine and tissue engineering. However, long-term in vitro culture of hBMMSCs leads to a reduced life span of the cells due to senescence, which leads eventually to growth arrest. To investigate the molecular mechanism behind the cellular senescence of hBMMSCs, microarray analysis was used to compare the expression profiles of early passage hBMMSCs, late passage hBMMSCs and hBMMSCs ectopically expressing human telomerase reverse transcriptase (hTERT). Using an intersection analysis of 3892 differentially expressed genes (DEGs) out of 27,171 total genes analyzed, we identified 338 senescence-related DEGs. GO term categorization and pathway network analysis revealed that the identified genes are strongly related to known senescence pathways and mechanisms. The genes identified using this approach will facilitate future studies of the mechanisms underlying the cellular senescence of hBMMSCs. ![]() |
![]() | Ex vivo expansion, adipogenesis and neurogenesis of cry... Cell Biology International |
![]() Cell Biology International, Volume 31, Issue 5, May 2007, Pages 444-450 Ying Xiang, Qiang Zheng, Bingbing Jia, Guoping Huang, Chungang Xie, Jianjun Pan, Jinfu Wang Abstract This study aimed to investigate the potentials of ex vivo expansion and pluridifferentiation of cryopreserved adult human bone marrow mesenchymal stem cells (hMSCs) into adipocytes and neurocytes. Cryopreserved hMSCs were resuscitated and cultured for 15 passages, and then induced to adipocytes and neurocytes with corresponding induction medium. The induced cells were observed for morphological properties and expression of triglyceride or neuron-specific enolase and nestin was detected. The result showed that the resuscitated cells cultured in induction medium consisting of dexamethasone, 3-isobutyl-1-methylxanthine, indomethacin and insulin-like growth factor I (IGF-I) showed adipogenesis, and lipid vacuole accumulation was detectable after 21 days. The resuscitated hMSCs were also induced into neurocytes and expressed nestin and neuron-specific enolase (NSE), which are special surface markers associated with neural cells at different stages. This study suggested that resuscitated hMSCs should still be a population of pluripotential cells and should be accessible for establishing an abundant hMSC reservoir for further experiment and treatment of various clinical diseases. ![]() |
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Behavioral improvement induced by bone marrow mesenchymal stem cells in neonatal rats after hypoxic-ischemic brain damage