The conditioned medium of murine and human adipose-derived stem cells exerts neuroprotective effects against experimental stroke model

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• 作者：Yusuke Egashira^a ; ^b ; Sou Sugitani^a ; Yukiya Suzuki^a ; Keisuke Mishiro^a ; Kazuhiro Tsuruma^a ; Masamitsu Shimazawa^a ; Shinichi Yoshimura^b ; Toru Iwama^b ; Hideaki Hara^a ; ^{hidehara@gifu-pu.ac.jp}
• 关键词：ASC ; adipose derived stem cell ; ASC-CM ; ASC-conditioned medium ; FBS ; fetal bovine serum ; MCAO ; middle cerebral artery occlusion ; MSC ; mesenchymal stem cells ; PBS ; phosphate-buffered saline
• 刊名：Brain Research
• 出版年：2012
• 期刊代码：8_00068993
• 类别：neu
• 出版时间：21 June, 2012
• 卷：1461
• 期：Complete
• 页码：87-95
• 文件大小：789 K

摘要

This study investigated the possible ameliorative effects of adipose-derived stem cells-conditioned medium (ASC-CM) on experimental ischemic stroke. In vivo ischemic stroke was induced in mice after 2 h of middle cerebral artery occlusion (MCAO) followed by 22 h reperfusion. Culture of SH-SY5Y human neuroblastoma cells with 100 渭M glutamate for 24 h was used as an in vitro neuronal apoptosis model. Intracerebroventricular (i.c.v.) administration of 30- and 100-fold concentrated murine ASC-CM 1 h prior to MCAO resulted in a dose-dependent reduction in the infarct volume and the brain swelling. The administration of murine ASC-CM immediately after MCAO was also effective, but administration 2 h after MCAO was not. Neuroprotective effects of murine ASC-CM were also confirmed in an in vitro model. Pretreatment with 100-fold concentrated murine ASC-CM at 10%of the total culture volume significantly reduced glutamate-induced excitotoxicity in the SH-SY5Y cells. Similar reduction in the MCAO-induced infarction volume was seen following i.c.v. administration of 100-fold concentrated human ASC-CM or murine ASC-CM. In conclusion, ASC-CM appears to exert ameliorative effects on experimental ischemic stroke i\n both in vivo and in vitro models. These findings suggest the feasibility of ASC-CM administration as a therapy for acute stage stroke.