Activation of NUDT5, an ADP-ribose pyrophosphatase, by nitric oxide-mediated ADP-ribosylation
- 作者：Hong-Nu Yu ; Eun-Kyung Song ; Seung-Min Yoo ; Young-Rae Lee ; Myung-Kwan Han ; Chang-Yeol Yim ; Jae-Yong Kwak ; Jong-Suk Kim
- 关键词：NUDT5 ; ADP-ribose pyrophosphatase ; ADP-ribosylation ; Nitric oxide ; Macrophage
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2007
- 期刊代码：159_0006291x
- 类别：bio
- 出版时间：16 March 2007
- 卷：354
- 期：3
- 页码：764-768
- 文件大小：179 K
摘要
The ADP-ribose (ADPR) pyrophosphatase (ADPRase) NUDT5, a member of a superfamily of Nudix hydrolases, hydrolyzes ADP-ribose (ADPR) to AMP and ribose 5′-phosphate. Nitric oxide (NO) enhances nonenzymatic ADP-ribosylation of proteins such as β-actin and glyceraldehydes 3-phosphate dehydrogenase in the presence of free ADPR, suggesting a possibility that NUDT5 could also be ADP-ribosylated by its substrate, ADPR. Here, we show that NO stimulates nonenzymatic ADP-ribosylation of NUDT5 using ADP-ribose and consequently activates its ADPRase activity. We found that ADPRase activity in J774 macrophage cells is increased by the treatment with SNP, an exogenous NO generator or TNF-α/IFN-γ, endogenous NO inducers. Anti-NUDT5 antibody pulled down most of the ADPRase activity increased by NO, indicating that the ADPRase regulated by NO is NUDT5. Using recombinant human NUDT5, we also demonstrated that the increase of ADPRase activity is mediated via ADP-ribosylation at cysteine residue(s) in the presence of reductant. This result suggests that NO activates NUDT5 through ADP-ribosylation at cysteine residues of the enzyme in macrophages.