Modeling bacteriophage attachment using adsorption efficiency

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• 作者：Zachary J. Storms^a ; Logan Smith^a ; Dominic Sauvageau^b ; ^{dominic.sauvageau@ualberta.ca} ; David G. Cooper^a
• 关键词：Bacteriophage ; Adsorption ; Adsorption efficiency ; Modeling ; Kinetic parameters ; Dynamic simulation
• 刊名：Biochemical Engineering Journal
• 出版年：2012
• 期刊代码：9_1369703x
• 类别：ce
• 出版时间：15 May, 2012
• 卷：64
• 期：Complete
• 页码：22-29
• 文件大小：539 K

摘要

Typically, models of bacteriophage adsorption consider the process in two steps: reversible and irreversible attachment. In this study, a recently introduced one-step adsorption model, the adsorption efficiency model, is used to describe the adsorption of T-series bacteriophages to Escherichia coli. The adsorption efficiency model simplifies phage attachment to a single step: irreversible binding. The adsorption efficiency (蓻) is used to account for unadsorbed phages. The model accurately describes T-series phage adsorption (T2, T4, T5, T6, and T7) under a variety of conditions. In addition, the model is compared to a commonly used two-step adsorption model, the sequential model. Experimental data support the assumptions of the adsorption efficiency model and suggest that the reversible first step of T-series phage adsorption is equivalent to irreversible attachment under the conditions tested. The adsorption efficiency model was not appropriate for a phage 位 strain lacking side tail fibers. However, the model did agree with data previously published for a strain of phage 位 possessing side tail fibers, as is the case of all T-series strains tested. This suggests that the adsorption efficiency model applies to phages containing side tail fibers